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首页> 外文期刊>Journal of Cellular Physiology >Differences in electron transport potential, antioxidant defenses, and oxidant generation in young and senescent fetal lung fibroblasts (WI-38).
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Differences in electron transport potential, antioxidant defenses, and oxidant generation in young and senescent fetal lung fibroblasts (WI-38).

机译：不同的电子传递的潜力,抗氧化防御系统,和氧化剂的一代年轻和衰老胎儿肺成纤维细胞(WI-38)。

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The activities and mRNA abundances of enzymes that regulate the rate of electron flow through the electron transport chain (ETC), including NADH dehydrogenase, succinate dehydrogenase, and cytochrome c oxidase, were examined in young and senescent fetal lung fibroblasts (WI-38). We also determined the activities and mRNA abundances of antioxidant defenses including superoxide dismutase, catalase, and glutathione peroxidase. We confirmed our previous report of a senescence-related increase in the abundance of ND4, a mitochondrially encoded subunit of NADH dehydrogenase. The activities of cytochrome c oxidase and NADH dehydrogenase were also elevated in senescent cultures. No differences were observed in the mRNA abundances of COX-1, a mitochondrially encoded subunit of cytochrome c oxidase or of nuclearly encoded subunits of various electron transport components (SD, COX-4, and ND 51). Lucigenin-detected chemiluminescence and H2O2 generation were both elevated in senescent cells. Catalase activity was also elevated in senescent fibroblasts. However, no differences in catalase mRNA abundance were observed. A small decrease in GSH peroxidase (GPx) mRNA abundance was observed in senescent cells. No other changes in the activities or mRNA abundances of any of the antioxidant defenses were observed in early and late passage cultures. The relationships between oxidant generation, mitochondrial enzyme activities, and antioxidant defense observed during proliferative senescence are dissimilar to those detected between fetal and postnatal fibroblasts as well as those found between fibroblast lines obtained from young and old individuals. The relevance of the differences between these models is discussed.
机译：酶的活动和mRNA丰度规范电子流过的速度电子传递链(等),包括NADH脱氢酶、琥珀酸脱氢酶和细胞色素c氧化酶,在年轻人和检查衰老胎儿肺成纤维细胞(WI-38)。确定活动和mRNA的丰度抗氧化防御系统包括超氧化物岐化酶、过氧化氢酶、谷胱甘肽过氧化物酶。我们证实了我们以前的报告伴随老化增加丰富ND4, NADH的线粒体编码单元脱氢酶。氧化酶和NADH脱氢酶也升高在衰老的文化。COX-1的观察mRNA的丰度线粒体细胞色素c的编码单元氧化酶或核编码的子单元各种电子传递组件(SD、COX-4和ND 51)。和过氧化氢生成都升高衰老细胞。在衰老成纤维细胞升高。过氧化氢酶的差异mRNA丰富观察到。(GPx) mRNA在衰老细胞。丰度的抗氧化防御系统观察在早期和晚期文化。氧化剂代之间的关系,线粒体酶活性和抗氧化剂国防增生性衰老期间观察到的胎儿之间是不同的检测和产后成纤维细胞以及这些发现从年轻而获得的纤维母细胞之间的线旧的个人。这些模型之间进行了讨论。

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