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首页> 外文期刊>Journal of Cellular Physiology >Mechanisms contributing to fluid-flow-induced Ca2+ mobilization in articular chondrocytes.
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Mechanisms contributing to fluid-flow-induced Ca2+ mobilization in articular chondrocytes.

机译：机制导致fluid-flow-induced Ca2 +在关节软骨细胞动员。

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摘要

We previously showed that fluid flow, which chondrocytes experience in vivo and which results in a variety of morphological and metabolic changes in cultured articular chondrocytes, can also stimulate a rise in intracellular calcium concentration ([Ca2+]i). However, the mechanism by which Ca2+ is mobilized in response to flow is unclear. In this study, we investigated the roles of intracellular Ca2+ stores, G-proteins, and extracellular ATP in the flow-induced Ca2+ response in bovine articular chondrocytes (BAC). Cells loaded with the Ca2+ sensitive dye Fura-2 were exposed to steady flow at 34 ml/min (37 dynes/cm2) in a parallel plate flow chamber. Whereas ryanodine and caffeine had no effect, both neomycin and thapsigargin significantly decreased the Ca2+(i) response to flow, suggesting a role for Ca2+ store release, possibly through an inositol 1,4,5-trisphosphate (IP3)-dependent mechanism. Twenty-four-hour treatment with pertussis toxin also significantly decreased the response, suggesting that the mechanism may be G-protein regulated. In addition, ATP release by chondrocytes does not appear to mediate the flow-induced Ca2+ response because suramin, a P2 purinergic blocker, had no effect. These results suggest that BAC respond rapidly to changes in their mechanical environment, such as increased fluid flow, by a mechanism that involves IP3 stimulated Ca2+(i) release and G-protein activation.
机译：我们先前表明,流体流动软骨细胞体内的经验和成果在各种各样的形态和代谢人工关节软骨细胞的变化,可以在细胞内的钙也刺激增长浓度([Ca2 +] i)。Ca2 +的动员来响应流不清楚。细胞内钙离子的商店,g细胞外ATP Ca2 +流反应牛关节软骨细胞(BAC)。细胞含有染料Fura-2 Ca2 +敏感暴露在稳定流34毫升/分钟(37达因/厘米2)在平行板流室。而利阿诺定和咖啡因没有影响,新霉素和thapsigargin显著减少Ca2 + (i)响应流,建议对Ca2 +存储发布的一个角色,可能通过一个肌醇1,4,5-trisphosphate(IP3)端依赖机制。治疗百日咳毒素也显著降低了反应,这表明机制可能是蛋白监管。此外,ATP释放由软骨细胞没有似乎调解Ca2 +流响应因为苏拉明,P2 purinergic杀杀杀,没有的效果。迅速变化的机械环境,如流体流动,增加了一个机制,包括IP3刺激Ca2 +(我)释放和g蛋白激活。

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