Nuclear translocation of mitogen-activated protein kinase p42MAPK during the ongoing cell cycle.

Hulleman E; Bijvelt JJ; Verkleij AJVerrips CTBoonstra J

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Nuclear translocation of mitogen-activated protein kinase p42MAPK during the ongoing cell cycle.

机译：核易位的增殖作用激酶p42MAPK期间持续的细胞周期。

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Mitogen-activated protein (MAP) kinases are serine/threonine protein kinases that are activated rapidly in cells stimulated by various extracellular signals. With stimulation of quiescent cells by growth factors, activated p42/p44 MAP kinases rapidly translocate to the nucleus, where they induce immediate early gene transcription. The MAP kinase signal transduction pathway represents an important mechanism by which growth factors regulate cellular events such as cell cycle progression or cell growth. In the present study, p42MAPK (ERK2) was studied during the ongoing cell cycle of Chinese hamster ovary cells synchronized by mitotic shake-off. We show that protein expression of p42MAPK increased in mid-G1 and that MAP kinase is phosphorylated during G1, as visualized by a gel-mobility shift and by the use of phosphospecific antibodies. This phosphorylation appeared to occur in the cytoplasm rather than at the plasma-membrane. In addition, phosphorylated p42MAPK was found to translocate to the nucleus during late/mid-G1. Treatment of cells with MEK inhibitor PD098059 prevented the phosphorylation and nuclear translocation of MAP kinase and DNA synthesis. Thus, nuclear translocation of p42MAPK is not restricted to the G0/G1 transition but occurs in every cell cycle and seems to be required for cell cycle progression.

机译：增殖作用(MAP)激酶丝氨酸/苏氨酸蛋白激酶迅速激活细胞受到各种刺激细胞外信号。静止的细胞生长因子,激活第42页/ p44激酶迅速把映射到核,引起立即早期基因转录。路径代表一个重要的机制生长因子调节细胞活动如细胞周期进展或细胞生长。目前的研究中,p42MAPK (ERK2)进行了研究在正在进行的中国仓鼠细胞周期卵巢细胞通过有丝分裂摆脱同步。表明p42MAPK蛋白表达增加在mid-G1 MAP激酶磷酸化在G1,可视化的gel-mobility转变通过使用phosphospecific抗体。这种磷酸化似乎发生在细胞质中,而不是在质膜。此外,磷酸化p42MAPK被发现把在晚/ mid-G1细胞核。治疗和MEK抑制剂PD098059细胞阻止了磷酸化和核易位的MAP激酶和DNA合成。因此,核易位p42MAPK不是局限于G0 / G1过渡,但发生在每一个细胞周期和似乎所需细胞周期进程。

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来源
《Journal of Cellular Physiology》 |1999年第3期|325-333|共9页
作者
Hulleman E; Bijvelt JJ; Verkleij AJVerrips CTBoonstra J;
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作者单位

Department of Molecular Cell Biology, Utrecht University, The Netherlands.;

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正文语种英语
中图分类细胞生物学;
关键词
Cell Cycle Progression; MAPK Signaling Pathway; Cell CycleGrowth FactorsMitogen-Activated Protein Kinase 1Mitogen-Activated Protein KinasesCell nucleusNuclear TranslocationNucleusCHO Cells;

机译：细胞周期进展、MAPK信号通路、细胞CycleGrowth FactorsMitogen-Activated蛋白质蛋白质KinasesCell激酶1增殖作用nucleusNuclear TranslocationNucleusCHO细胞;

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Nuclear translocation of mitogen-activated protein kinase p42MAPK during the ongoing cell cycle.

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