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首页> 外文期刊>Journal of Cellular Physiology >Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells.
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Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells.

机译：表型和功能属性的人类骨髓间充质祖细胞。

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摘要

Bone marrow stroma provides the microenvironment for hematopoiesis and is also the source of mesenchymal progenitors (mesenchymal or marrow stromal cells [MSC]) that may serve as long-lasting precursors for bone, cartilage, lung, and muscle. While several studies have indicated the differentiation potential of MSC, few studies have been performed on the cells themselves. In an attempt to further expand our knowledge on these cells, we have performed studies on their cell cycle, immuno- and adhesive-phenotype, ex vivo expansion, and differentiation properties. MSC cultures have been initiated from human bone marrow low-density mononuclear cells and maintained in the absence of differentiation stimuli and hematopoietic cells. The homogenous layer of adherent cells thus formed exhibits a typical fibroblastlike morphology, a population doubling time of 33 h, a large expansive potential, and cell cycle characteristics including a subset (20%) of quiescent cells. The antigenic phenotype of MSC is not unique, borrowing features of mesenchymal, endothelial, and epithelial cells. Together, MSC express several adhesion-related antigens, like the integrin subunits alpha4, alpha5, beta1, integrins alphavbeta3 and alphavbeta5, ICAM-1, and CD44H. MSC produce and functionally adhere to extracellular matrix molecules. When incubated under proper stimuli, MSC differentiate into osteoblasts or adipocytes. Taken together, these results demonstrate that adherent marrow-derived cells cultured in the absence of hematopoietic cells and differentiation stimulus give rise to a population of cells with phenotypical and functional features of mesenchymal progenitors. The existence of a subset of quiescent cells in MSC cultures seems to be extremely significant, since their number and properties should be enough to sustain a steady supply of cells that upon proliferation and commitment may serve as precursors for a number of nonhematopoietic tissues. Copyright 1999 Wiley-Liss, Inc.
机译：骨髓间质提供了微环境造血作用和也的来源间叶细胞祖细胞(间叶细胞或骨髓基质细胞(MSC)),可以作为持久的前体为骨骼、软骨、肺、肌肉。表示MSC分化的潜力,一些研究已经进行细胞他们自己。这些细胞知识,我们的表现研究细胞周期、免疫和adhesive-phenotype、体外扩张分化特性。从人类开始骨髓低密度在缺乏单核细胞和维护分化的刺激和造血细胞。从而形成了一个典型fibroblastlike展品形态、33 h的人口倍增时间大的潜力,和细胞周期特征包括(20%)的一个子集静止的细胞。不是独一无二的,间叶细胞的借贷特征,内皮和上皮细胞。表达几个adhesion-related抗原,如整合素亚基alpha4、alpha5 beta1,整合蛋白alphavbeta3 alphavbeta5, ICAM-1,和CD44H。细胞外基质分子。在适当的刺激下,MSC分化成成骨细胞、脂肪细胞。结果表明,附着骨髓来源在缺乏造血细胞培养细胞和分化刺激引起细胞表型和人口间叶细胞祖细胞的功能特性。静止细胞的一个子集的存在MSC文化似乎是非常重要的,因为他们的数量和属性足以维持细胞的稳定供应可以作为扩散和承诺前体的nonhematopoietic组织。

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