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首页> 外文期刊>Journal of Cellular Physiology >Regulation of myelin basic protein gene transcription by Sp1 and Puralpha: evidence for association of Sp1 and Puralpha in brain.
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Regulation of myelin basic protein gene transcription by Sp1 and Puralpha: evidence for association of Sp1 and Puralpha in brain.

机译：髓磷脂碱性蛋白基因的调控转录Sp1和Puralpha:证据协会Sp1和Puralpha大脑。

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Direct interaction between transcription factors may provide a mechanism for the regulatory function of these proteins on transcription of the responsive genes. These interactions may be facilitated if the target DNA sequences for the participant regulatory proteins are overlapped or positioned in close proximity to each other within the promoter of the responsive genes. In earlier studies, we identified a cellular protein, named Puralpha, which upon binding to the MB1 regulatory DNA sequence of the myelin basic protein (MBP) gene, stimulates its transcription in central nervous system (CNS) cells. Here, we provide evidence for binding of the ubiquitous DNA binding transcription factor, Sp1, to the MB1 DNA motif at the region that partially overlaps with the Puralpha binding site. We demonstrate that binding of Puralpha to its target sequence is enhanced by inclusion of Sp1 in the binding reaction. Under this condition, binding of Sp1 to the MB1 regulatory sequence remained fairly unchanged, and no evidence for the formation of Puralpha:MB1:Sp1 was observed. This observation suggests that transient interaction of Puralpha and Sp1 may result in stable association of Puralpha and the MB1 element. In support of this notion, results from immunoprecipitation/Western blot studies have established association of Puralpha and Sp1 in nuclear extracts from mouse brain. Of interest, Puralpha appears to bind to the phosphorylated form of Sp1 which is developmentally regulated and that coincides with the periods when MBP gene expression is at its maximum level. Results from cotransfection studies revealed that ectopic expression of Puralpha and Sp1 synergistically stimulates MBP promoter activity in CNS cells. The importance of these findings in stage-specific expression of MBP during brain development is discussed. Copyright 1999 Wiley-Liss, Inc.
机译：转录因子之间的直接交互可以提供一个机制来监管呢这些蛋白质的转录功能响应基因。促进目标的DNA序列参与者监管重叠或蛋白质定位在靠近对方在响应基因的启动子。早期的研究,我们发现了一个细胞蛋白质,名叫Puralpha,绑定MB1监管髓磷脂的DNA序列碱性蛋白(MBP)基因,刺激它在中枢神经系统(CNS)转录细胞。无处不在的DNA结合转录因子,Sp1, MB1 DNA主题区域部分重叠的Puralpha绑定网站。其目标序列是由包含增强Sp1的结合反应。条件,绑定MB1 Sp1的监管序列保持相对不变,没有证据的形成Puralpha: MB1: Sp1被观察到。瞬态交互Puralpha和Sp1导致Puralpha协会和稳定MB1元素。从免疫沉淀反应/免疫印迹的研究建立了Puralpha协会和Sp1吗在核提取物老鼠的大脑。兴趣,Puralpha似乎绑定到磷酸化的Sp1发育调控,正值时候MBP基因表达的最高水平。研究表明,异位表达的Puralpha和Sp1协同刺激MBP在中枢神经系统细胞启动子活动。这些发现在stage-specific表达MBP讨论在大脑发育。版权1999 Wiley-Liss公司。

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