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首页> 外文期刊>Journal of Cellular Physiology >Protein kinase C activator PMA reduces the Ca(2+) response to antigen stimulation of adherent RBL-2H3 mucosal mast cells by inhibiting depletion of intracellular Ca(2+) stores.
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Protein kinase C activator PMA reduces the Ca(2+) response to antigen stimulation of adherent RBL-2H3 mucosal mast cells by inhibiting depletion of intracellular Ca(2+) stores.

机译：蛋白激酶C的激活PMA减少Ca (2 +)抗原刺激的附着RBL-2H3粘膜肥大细胞通过抑制消耗细胞内的钙(2 +)商店。

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Activation of protein kinase C has been shown to reduce the Ca(2+) responses of a variety of cell types. In most cases, the reduction is due to inhibition of Ca(2+) influx, but acceleration of Ca(2+) efflux and inhibition of Ca(2+) store depletion by protein kinase C activation have also been described. For adherent RBL-2H3 mucosal mast cells, results from whole-cell patch clamp experiments suggest that protein kinase C activation reduces Ca(2+) influx, while experiments with intact, fura-2-loaded cells suggest that Ca(2+) influx is not affected. Here we present single-cell data from Ca(2+) imaging experiments with adherent RBL-2H3 cells, showing that antigen-stimulated Ca(2+) responses of phorbol 12-myristate 13-acetate (PMA)-treated cells are more transient than those of control cells. PMA also reduced the response to antigen in the absence of extracellular Ca(2+), indicating that depletion of intracellular Ca(2+) stores is inhibited. If PMA was added after stores had been depleted by thapsigargin, a small decrease in [Ca(2+)](i) was observed, consistent with a slight inhibition of Ca(2+) influx. However, the major effect of PMA on the antigen-stimulated Ca(2+) response is to inhibit depletion of intracellular Ca(2+) stores. We also show that inhibition of protein kinase C did not enhance the Ca(2+) response to antigen, suggesting that inhibition of the Ca(2+) response by activation of protein kinase C does not contribute to the physiological response to antigen. Copyright 1999 Wiley-Liss, Inc.
机译：激活蛋白激酶C已被证明减少Ca(2 +)反应的各种细胞类型。抑制钙(2 +)流入,但加速Ca(2 +)流出和抑制Ca(2 +)存储损耗由蛋白激酶C的激活也被描述。从全细胞膜片箝肥大细胞,结果实验表明,蛋白激酶C激活减少Ca(2 +)流入,而实验与完整,fura-2-loaded细胞表明,Ca(2 +)流入不受影响。我们现在的单细胞Ca(2 +)成像的数据与附着RBL-2H3细胞实验,显示antigen-stimulated Ca(2 +)的反应佛波醇12-myristate 13-acetate (PMA)治疗细胞比那些瞬态控制细胞。在缺乏细胞外钙(2 +),表明细胞内钙的消耗(2 +)商店受到抑制。小商店被thapsigargin耗尽减少(Ca(2 +))(我)所观察到的,一致的有轻微抑制Ca(2 +)涌入。然而,PMA的重大影响antigen-stimulated Ca(2 +)反应是抑制消耗细胞内的钙(2 +)商店。表明,抑制蛋白激酶C没有提高Ca(2 +)反应抗原,表明抑制Ca(2 +)的反应通过激活蛋白激酶C不导致的生理反应抗原。

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