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首页> 外文期刊>Journal of Cellular Physiology >Phosphatidylinositol- and phosphatidylcholine-dependent phospholipases C are involved in the mechanism of action of atrial natriuretic factor in cultured rat aortic smooth muscle cells.
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Phosphatidylinositol- and phosphatidylcholine-dependent phospholipases C are involved in the mechanism of action of atrial natriuretic factor in cultured rat aortic smooth muscle cells.

机译：磷脂酰肌醇,phosphatidylcholine-dependent磷脂酶C参与的作用机制心房利钠因子在培养大鼠主动脉光滑肌肉细胞。

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摘要

We have investigated the involvement of specific phospholipase systems and their possible mutual relationship with the mechanism by which atrial natriuretic factor (ANF) increases phosphatidate (PA) and diacylglycerol (DAG) in rat aortic smooth muscle cells (RASMC), one of the major targets of this hormone. Our results indicate that ANF initially stimulates a phosphatidylinositol-dependent phospholipase C (PI-PLC) with a significant increase of DAG, enriched in arachidonate, and inositol trisphosphate (IP3) and then a phosphatidylcholine-dependent phospholipase C (PC-PLC) with formation of DAG, enriched in myristate, and phosphocholine (Pcho). Moreover, ANF stimulates PA formation at an intermediate stage between early and late DAG formation. The transphosphatidylation reaction, as well as its labeling ratio, demonstrate that phosphatidylcholine-dependent phospholipase D (PC-PLD) is not involved. Our experiments with R59022, a DAG kinase (DAGK) inhibitor, indicate that such an increase may be due to the phosphorylation of DAG derived from phosphatidylinositol (PI) hydrolysis. Our results show that phorbol 12-myristate 13 acetate (PMA) plays a significant role in late DAG formation and that Pcho is released concomitantly, suggesting there is a relationship between the two phospholipase Cs (PLCs) that occurs through a protein kinase C (PKC) translocation from cytosol to the plasma membrane. These findings are confirmed by the use of PKC inhibitors calphostin, H7, and staurosporine. The involvement of membrane phospholipid hydrolysis and the ensuing production of second messengers might explain the vasorelaxant effect of ANF.
机译：我们有具体的参与调查磷脂酶系统和其可能的相互的与心房的机制之间的关系利钠因子(曾帮工)增加phosphatidate(PA)和甘油二酯在大鼠主动脉(DAG)平滑肌细胞(RASMC)的一个专业这种激素的目标。曾帮工最初刺激phosphatidylinositol-dependent磷脂酶C(PI-PLC) DAG的显著增加,丰富的花生四烯酸和肌醇联结(IP3),然后phosphatidylcholine-dependent磷脂酶C(PC-PLC) DAG的形成,丰富十四烷酸和胆碱磷酸(Pcho)。曾帮工刺激PA形成中间阶段之间的早期和晚期DAG的形成。transphosphatidylation反应,以及它的标签比,证明phosphatidylcholine-dependent磷脂酶D(PC-PLD)并没有参与进来。R59022, DAG激酶抑制剂(DAGK)表示这样的增加可能是由于磷酸化的DAG来自磷脂酰肌醇(PI)水解。表明,佛波醇醋酸12-myristate 13 (PMA)在DAG末形成中扮演一个重要的角色与此同时,Pcho释放,建议有关系两个磷脂酶c (plc)通过一个发生从胞质蛋白激酶C (PKC)易位质膜。证实了PKC抑制剂的使用calphostin、H7和staurosporine。参与膜磷脂水解和随后的生产的第二信使或许可以解释曾帮工的vasorelaxant效果。

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