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首页> 外文期刊>Journal of Cellular Physiology >Prostaglandins stimulate the stress-induced synthesis of hsp27 and alpha B crystallin.
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Prostaglandins stimulate the stress-induced synthesis of hsp27 and alpha B crystallin.

机译：前列腺素刺激应激hsp27和αB晶状体蛋白的合成。

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The effects were examined of various prostaglandins (prostaglandin A1, A2, J2, E2, and D2) on the stress-induced accumulation of hsp27 and alpha B crystallin in C6 rat glioma cells. The levels of hsp27 and alpha B crystallin, which were determined by specific immunoassays, were low in cells in confluent cultures. The levels of the two proteins increased after exposure of cells to heat (42 degrees C for 30 min) or arsenite (50 microM for 1 h). Cells exposed to 10 microM each of prostaglandin A1, A2, or J2 for 1 h resulted in stimulation of the binding to the heat shock element (HSE) of heat shock transcription factor (HSF). However, there was no phosphorylation-dependent mobility shift of HSF1 and no subsequent increase in the transcription and translation for hsp27, alpha B crystallin, and hsp70. When cells were exposed to arsenite in the presence of 10-40 microM prostaglandin, the accumulation of hsp27 and alpha B crystallin in cells was enhanced markedly. The levels of hsp70 also increased in cells that had been treated with arsenite in the presence of a prostaglandin, as estimated by Western blot analysis. Northern blot analysis revealed that the expression of messenger RNAs (mRNAs) for hsp27, alpha B crystallin, and hsp70 was enhanced in cells that had been exposed to arsenite in the presence of each prostaglandin. Similar stimulatory effects of prostaglandins also were observed in the case of the heat-induced responses of hsp27, alpha B crystallin, and hsp70. Gel mobility shift assays revealed that each prostaglandin prolonged the arsenite-induced binding of HSF to HSE. These results suggest that the pharmacological dose of prostaglandins stimulates the stress-induced synthesis of stress proteins via activation of the HSF.
机译：各种检查的影响前列腺素(前列腺素A1, A2, J2、E2和D2)应激hsp27的积累和αB晶状体蛋白C6鼠胶质瘤细胞。hsp27和αB晶状体蛋白的水平,取决于具体的分析,是吗低细胞融合性的文化。暴露后的两种蛋白质增加细胞热(30分钟42摄氏度)或亚砷酸(50 microM 1 h)。细胞暴露于10microM每个前列腺素A1, A2,或J2 1h导致刺激的绑定热休克元素(HSE)的热休克转录因子(HSF)。phosphorylation-dependent HSF1的流动转变没有后续增加转录和翻译hsp27、αB晶状体蛋白,和hsp70。的存在10-40 microM前列腺素,hsp27积累和αB晶状体蛋白细胞显著增强。也增加了在细胞治疗亚砷酸与前列腺素的存在,所估计的免疫印迹分析。污点分析显示的表达信使核糖核酸(mrna) hsp27、αB晶状体蛋白,hsp70在细胞增强被暴露于亚砷酸盐的存在吗每个前列腺素。前列腺素也观察到的情况hsp27的燥热引起的反应,αB晶状体蛋白,hsp70。显示每个前列腺素延长了arsenite-induced绑定HSF HSE。结果表明,药理剂量的前列腺素刺激应激通过激活应力合成蛋白质HSF。

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