Possible role of coexpression of CD9 with membrane-anchored heparin-binding EGF-like growth factor and amphiregulin in cultured human keratinocyte growth.

Inui S; Higashiyama S; Hashimoto KHigashiyama MYoshikawa KTaniguchi N

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Possible role of coexpression of CD9 with membrane-anchored heparin-binding EGF-like growth factor and amphiregulin in cultured human keratinocyte growth.

机译：可能的coexpression CD9的角色membrane-anchored heparin-binding EGF-like增长因素和amphiregulin培养人角化细胞生长。

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CD9 is a protein with 4 transmembrane domains, and functions as a cell surface antigen. We have previously reported that CD9 functions as an up-regulator of membrane-anchored heparin-binding EGF-like growth factor (proHB-EGF) activity, which is a potent mitogen as well as a soluble HB-EGF. Anti-CD9 antibodies can neutralize the juxtacrine activity of proHB-EGF when both CD9 and proHB-EGF are coexpressed. We demonstrated here: (1) the CD9 gene was transcribed and translated in the cultured human keratinocytes; (2) anti-CD9 antibody inhibited the approximately 50% growth of human keratinocytes in culture; (3) CD9 was coprecipitated with proHB-EGF and membrane-anchored amphiregulin (proAR), and (4) the transient coexpression of CD9 with proHB-EGF or proAR in mouse L cells up-regulated their juxtacrine growth factor activities. These results suggest that CD9 would make a heterodimer and/or trimer complex with proHB-EGF and proAR, and might cooperate with proHB-EGF and proAR for human keratinocyte growth ina juxtacrine manner.

机译：与4跨膜域CD9是一种蛋白质,函数作为细胞表面抗原。此前报道称,作为CD9功能的up-regulator membrane-anchored heparin-bindingEGF-like生长因子(proHB-EGF)活动,这是一个强大的有丝分裂原以及可溶性HB-EGF。当两个CD9 juxtacrine proHB-EGF活动和proHB-EGF coexpressed。: (1) CD9基因转录和翻译在人类角质细胞培养;(2) anti-CD9抗体抑制了约增长50%的人类角质细胞在文化;CD9和proHB-EGF和共沉淀membrane-anchored amphiregulin (proAR),和(4)CD9的瞬态coexpression proHB-EGF或proAR老鼠L细胞上调他们的juxtacrine生长因子的活动。结果表明,CD9将异质二聚体和/或三聚物复杂proHB-EGF和proAR,可能与proHB-EGF和proAR合作人类角质细胞生长在juxtacrine方式。

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来源
《Journal of Cellular Physiology》 |1997年第3期|291-298|共8页
作者
Inui S; Higashiyama S; Hashimoto KHigashiyama MYoshikawa KTaniguchi N;
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作者单位

Department of Biochemistry, Osaka University Medical School, Japan.;

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原文格式 PDF
正文语种英语
中图分类细胞生物学;
关键词
Surface Antigens; Heparin-binding EGF-like Growth Factor; Growth SubstancesKeratinocytesGrowth FactorsAmphiregulinantigen p24Glycoproteins;

机译：表面抗原;Heparin-binding EGF-like增长因素;增长SubstancesKeratinocytesGrowthFactorsAmphiregulinantigen p24Glycoproteins;

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1. A metalloprotease-disintegrin, MDC9/meltrin-gamma/ADAM9 and PKCdelta are involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. [J] . Izumi Y, Hirata M, Hasuwa HIwamoto RUmata TMiyado KTamai YKurisaki TSehara-Fujisawa AOhno SMekada E EMBO Journal . 1998,第24期

机译：metalloprotease-disintegrin,MDC9 / meltrin-gamma ADAM9和PKCdelta参与TPA-induced ectodomain脱落membrane-anchored heparin-binding EGF-like增长的因素。
2. Differential effects of a heparin antagonist (hexadimethrine) or chlorate on amphiregulin, basic fibroblast growth factor, and heparin-binding EGF-like growth factor activity. [J] . Cook PW, Ashton NM, Karkaria CESiess DCShipley GD Journal of Cellular Physiology . 1995,第2期

机译：微分作用的肝素拮抗剂amphiregulin (hexadimethrine)或氯酸盐,基本的纤维母细胞生长因子,和heparin-binding EGF-like生长因子的活动。
3. Studies on the role of membrane conductance changes in electrolyte secretion and volume regulation in the cultured rat and human epididymal cells. [O] . 1993

机译：studies on the role of membrane conductance changes in electrolyte secretion and volume regulation in the cultured rat and human epididymal cells.

Possible role of coexpression of CD9 with membrane-anchored heparin-binding EGF-like growth factor and amphiregulin in cultured human keratinocyte growth.

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