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首页> 外文期刊>Journal of Cellular Physiology >Cell behavior and cell-matrix interactions of human palmar aponeurotic cells in vitro.
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Cell behavior and cell-matrix interactions of human palmar aponeurotic cells in vitro.

机译：细胞的行为和cell-matrix交互人掌腱膜的细胞在体外。

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摘要

The present investigation has been performed to better characterize, in vitro, normal aponeurotic cells in comparison with dermal fibroblasts and with cells derived from Dupuytren's affected aponeuroses. Cells were cultured in monolayer and/or into three-dimensional collagen gels. Cell structure, adhesion, and spreading capability on different substrates, as well as integrin expression were investigated by light and electron microscopy and by flow cytometry. Cell-matrix interactions were also analyzed by gel retraction experiments in the presence, or absence, of RGD peptides and anti-integrin antibodies. Normal aponeurotic cells, compared with dermal fibroblasts, exhibited in vitro peculiar structural features, which were substantially maintained in Dupuytren's aponeurotic cells, irrespective of the substrate they were grown on. By contrast, the aponeurotic cell behavior was different in normal and diseased cells, these latter approaching that of dermal fibroblasts. Normal aponeurotic cells, in fact, were characterized by low efficiency in retracting the collagen gel, low alpha 2, alpha 1, and alpha 5 integrin subunit expression and low adhesion properties onto collagen and fibronectin, whereas cells isolated from the aponeuroses of Dupuytren's patients exhibited higher capability of retracting the collagen gel, increased adhesion properties toward collagen and fibronectin, and higher levels of integrin expression. No differences were observed between dermal fibroblasts from Dupuytren's patients or from normal subjects. These in vitro results are consistent with those previously obtained in situ, suggesting that palmar aponeurotic cells have a peculiar phenotype and that changes in cell-matrix interactions occur in Dupuytren's contracture. Moreover, by comparing data obtained from the retracted fibrotic cords and the still clinically unaffected aponeuroses of the same patients, it may be noted that Dupuytren's disease is not only confined to the clinically involved branches, but includes the whole aponeurosis of the affected hand.
机译：目前的调查已经完成更好的描述,在体外,正常的腱膜的真皮成纤维细胞与细胞与细胞来源于Dupuytren的影响腱膜。和/或成三维胶原凝胶。结构、附着力和传播能力不同的基质,以及整合素表达式被光和调查电子显微镜和流式细胞术。也分析了Cell-matrix交互在凝胶收缩实验,或没有,RGD肽和anti-integrin抗体。真皮成纤维细胞,在体外展出特殊的结构特点,大幅Dupuytren的维护腱膜的细胞,不考虑底物他们生长在。在正常细胞行为是不同的病变的细胞,这些后者接近的真皮成纤维细胞。事实上,在低效率的特征收回胶原凝胶、低α,α1,α5整合素亚基的表达到胶原蛋白和低附着力属性纤连蛋白,而细胞分离腱膜Dupuytren的病人表现出更高的能力收回胶原凝胶,对胶原蛋白和增加附着力属性纤连蛋白和更高水平的整合素表达式。从Dupuytren病人或真皮成纤维细胞从正常的受试者。与那些先前获得的一致原地,这表明掌腱膜的细胞有一个特殊的表型和变化cell-matrix交互发生在Dupuytren的挛缩。从收回的纤维化和绳索临床上的腱膜的影响病人,可能会指出,Dupuytren的不仅局限于临床疾病涉及的分支,但包括整个腱膜的影响。

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