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首页> 外文期刊>Journal of Cellular Physiology >Factor X-dependent, thrombin-generating activities on a neuroblastoma cell and their disappearance upon differentiation.
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Factor X-dependent, thrombin-generating activities on a neuroblastoma cell and their disappearance upon differentiation.

机译：因素X-dependent thrombin-generating活动在神经母细胞瘤细胞和他们的消失在分化。

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Some tumor cells induce platelet aggregation in the bloodstream, which has been implicated in tumor metastasis. In this study, we investigated the mechanism of platelet aggregation induced by a human neuroblastoma cell line, GOTO. It was revealed that GOTO cells had tissue factor on their surface and converted factor X (FX) to FXa with the aid of factor VIIa. The produced FXa formed prothrombinase complex on the cells and activated prothrombin. From experiments on activity inhibition by specific monoclonal as well as polyclonal antibodies, it was concluded that factor V did not constitute this prothrombinase complex. Another cofactor known to constitute prothrombinase complex on some cells, effector cell protease receptor-1 (EPR-1), was not expressed on GOTO cells, suggesting that the cofactor composing FXa-dependent prothrombinase activity on GOTO cells is not factor V or EPR-1 but, rather, is an unknown molecule. Upon the culturing in the presence of 5-bromo-2'-deoxyuridine for 4 days, GOTO cells differentiated into Schwann-like cells, and both FXase and prothrombinase activities were greatly diminished. Flow cytometric analyses revealed that the decrease of FXase activity should be attributed to the decrease of tissue factor expression on GOTO cells. Because these activities greatly diminished upon cellular differentiation, the expression of both cofactor molecules may be related to the malignant and metastatic nature of the tumor cells.
机译：一些肿瘤细胞诱导血小板聚集已经涉及到血液中肿瘤的转移。引起的血小板聚集的机理人类神经母细胞瘤细胞系,转到。透露,转到细胞组织因子表面和转换因子X——(外汇)借助VIIa因素。在细胞和形成prothrombinase复杂凝血酶原激活。通过特定的单克隆活动抑制多克隆抗体,这是结论这个因子V不构成prothrombinase复杂。构成prothrombinase复杂一些细胞,效应细胞蛋白酶receptor-1 (EPR-1)不转到细胞上表达,这表明代数余子式组合FXa-dependent prothrombinase活动不是因子V或EPR-1 GOTO细胞,而是是一个未知的分子。培养的5-bromo-2的脱氧尿苷4天,转到细胞分化成Schwann-like细胞,两者兼而有之FXase prothrombinase活动极大地减少了。FXase活动的减少归因于组织因子的减少表情GOTO细胞。在细胞活动大大降低分化,两个代数余子式的表达分子可能与恶性和有关转移性肿瘤细胞的性质。

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