Maitotoxin, a calcium channel activator, inhibits cell cycle progression through the G1/S and G2/M transitions and prevents CDC2 kinase activation in GH4C1 cells.

Van Dolah FM; Ramsdell JS

首页> 外文期刊>Journal of Cellular Physiology >Maitotoxin, a calcium channel activator, inhibits cell cycle progression through the G1/S and G2/M transitions and prevents CDC2 kinase activation in GH4C1 cells.

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Maitotoxin, a calcium channel activator, inhibits cell cycle progression through the G1/S and G2/M transitions and prevents CDC2 kinase activation in GH4C1 cells.

机译：Maitotoxin,钙通道激活剂,抑制细胞周期进程通过G1 / S和G2 / M转换和防止CDC2激酶激活在GH4C1细胞。

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Calcium regulates progression through several checkpoints in the cell cycle, including the G1/S-phase transition, G2/M-phase transition, and exit from mitosis. In the GH4C1 rat pituitary cell line, calcium mobilizing polypeptides and calcium channel activation inhibit cell proliferation. This report examines the effects of maitotoxin (MTX), an activator of type L voltage-dependent calcium channels (L-VDCC), on calcium influx and cell cycle progression in GH4C1 cells. MTX causes both a block from G1 to S-phase and a concentration-dependent accumulation of cells in G2+M. MTX does not increase the mitotic index; thus, sustained calcium channel activation by MTX results in an accumulation of cells in G2. In order to temporally localize the MTX-induced G2 block relative to cell cycle regulatory events at the G2/M transition, we assessed the relative activity of two cell cycle regulatory protein kinases, CDC2 and CDK2, in MTX-treated cells. CDC2-specific histone kinase activity in MTX-treated cells is lower than either in cells blocked in mitosis with the microtubule destabilizing agent demecolcine or in randomly cycling cells. In contrast, the activity of CDK2 is highest in MTX-treated cells, consistent with a G2 block prior to CDC2 activation. Together, these results implicate with a G2 block prior to CDC2 activation. Together, these results implicate calcium as an intracellular signal required for progression through G2 phase of the cell cycle prior to CDC2 kinase activation.

机译：通过几个钙调节过程在细胞周期检查点,包括G1 / s阶段过渡,G2 / m期过渡,和有丝分裂退出。细胞系,钙动员多肽和钙通道激活抑制细胞扩散。maitotoxin (MTX), L型的催化剂压敏电阻器钙通道(L-VDCC)钙流入和细胞周期进程GH4C1细胞。s阶段和浓度积累细胞G2 + M。增加细胞有丝分裂指数;钙通道激活MTX的结果在G2积累的细胞。暂时本地化MTX-induced G2的块相对于细胞周期的监管活动G2 / M过渡,我们评估了相对的两个细胞周期调控蛋白的活性激酶,CDC2、CDK2 MTX-treated细胞。CDC2-specific组蛋白激酶活性MTX-treated细胞低于在细胞阻塞与微管在有丝分裂不稳定剂秋水仙胺或随机骑自行车的细胞。最高MTX-treated细胞,符合吗G2块CDC2之前激活。这些结果表明G2块之前CDC2激活。涉及作为细胞内钙信号需要通过G2期的进展细胞周期之前CDC2激酶激活。

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来源
《Journal of Cellular Physiology》 |1996年第1期|49-56|共8页
作者
Van Dolah FM; Ramsdell JS;
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作者单位

Marine Biotoxins Program, Charleston Laboratory, U.S. National Marine Fisheries Service, Charleston, South Carolina 29412, USA.;

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正文语种英语
中图分类细胞生物学;
关键词
Cell Cycle Progression; KIAA1549L gene; MitosisS PhaseMarine ToxinsCalcium ChannelsmaitotoxinCOPPER ALLOY NO 92310CDC2 Protein KinaseCell CycleCell Division Kinase 2Calcium Channel AgonistsCRYGEP genePRRC2A gene;

机译：细胞周期进展;KIAA1549L基因;有丝分裂PhaseMarine ToxinsCalcium没有92310 cdc2 ChannelsmaitotoxinCOPPER合金蛋白质KinaseCell CycleCell分裂激酶2钙通道AgonistsCRYGEP genePRRC2A基因;

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Maitotoxin, a calcium channel activator, inhibits cell cycle progression through the G1/S and G2/M transitions and prevents CDC2 kinase activation in GH4C1 cells.

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