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首页> 外文期刊>Journal of Cellular Physiology >Quantitative analysis of collagen gel contractile forces generated by dermal fibroblasts and the relationship to cell morphology.
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Quantitative analysis of collagen gel contractile forces generated by dermal fibroblasts and the relationship to cell morphology.

机译：胶原凝胶收缩的定量分析由真皮成纤维细胞和力量与细胞形态的关系。

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The force generated in granulation tissue during wound contraction is thought to be cell mediated; however, it is unclear whether contractile forces are generated by fibroblast locomotion or contraction of myofibroblasts. To help clarify this question the force of this contraction can now be determined accurately in a human dermal fibroblast collagen lattice system using a novel instrument known as a Culture Force Monitor. Three distinct phases of contraction of such collagen gels could be identified over the first 24 hours. Most of the force generated by human dermal fibroblasts was produced during the first stage in parallel with cell attachment and associated changes in cell shape, and the appearance of cell processes. During this initial 24 hours no evidence could be found for the presence of myofibroblasts, but stereoscopic and electron microscopic analysis at a range of time points indicated that migratory fibroblasts were present in the system. Comparison of the contraction profiles of cells extracted from other tissues (tendon and articular cartilage), and extracted by different means from the same tissue specimen, indicated that different populations of fibroblasts can be distinguished on the basis of their pattern of contractions. It would seem that most of the force generated in this model is a result of fibroblast attachment and movement within the collagen lattice. Furthermore, different groups of fibroblasts, even within the same tissue, may vary in their contraction (hence locomotory) activity.
机译：在肉芽组织生成的力伤口收缩被认为是细胞介导;然而,目前尚不清楚收缩力量是由纤维母细胞生成运动还是myofibroblasts收缩。这个问题这收缩的力量现在准确地确定在一个人类的皮肤成纤维细胞胶原晶格系统使用一个小说监测仪器被称为一种文化力量。三个不同的阶段的收缩胶原蛋白凝胶可以确定在第一24小时。真皮成纤维细胞是在第一次生产与细胞的依恋和并行阶段相关的细胞形状的变化,外观的细胞过程。24小时没有能找到证据myofibroblasts面前,但立体和电子显微镜分析的范围点表示,迁徙的成纤维细胞现在系统中。收缩的细胞中提取的其他组织(肌腱和关节软骨),并从相同的提取的方式不同组织标本,表明不同成纤维细胞的数量可以区分的基础上的模式,收缩。似乎大部分的力量中产生这个模型是成纤维细胞的结果附件在胶原晶格和运动。此外,不同组的成纤维细胞,即使在相同的组织,可能会有所不同收缩(因此运动的)活动。

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