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首页> 外文期刊>Journal of Cellular Physiology >Persistent effects of phorbol 12-myristate 13-acetate: possible implication of vesicle traffic.
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Persistent effects of phorbol 12-myristate 13-acetate: possible implication of vesicle traffic.

机译：佛波12-myristate持久的影响13-acetate:泡的可能影响流量。

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Relative to their normal counterparts, transformed epithelial cells have a distinctive and quantifiable three-dimensional shape. Biophysical and mathematical methods are used to distinguish these extremes in cells from two lines, cultured from rat liver and tracheal epithelium, respectively. Cells adopted a more transformed-looking configuration transiently when exposed to phorbol 12-myristate 13-acetate (PMA) (Plummer and Heckman, [1990] Exp. Cell Res., 188:66-74). The purpose of the present work was to dissect the physiological processes involved in the shape change. Ruffling activity, known to be PMA-stimulated in other cells, was investigated. Although the ruffles appeared less robust than normal, PMA stimulated ruffling activity over a 5 h period. The number of sites where ruffling was initiated declined by 5 h, however, and suppression was seen by 10 h. Cells from both lines adopted the transformed shape configuration when exposed for 2 h to monensin. When the subset of shape features changed by this treatment was compared with those originally changed during transformation, it was found that monensin-treated cells mimicked the features of transformed cells. Its effect on ruffling was, however, unlike PMA's. Thus, the phenotype was unlikely to arise from ruffling itself but might be a process driven by ruffling. Chloroquine also stimulated cells to assume characteristics of transformed cells. Since both it and monensin could interfere with endosomes and with the processing of endocytosed contents, this was a likely site of action. Experiments were done to determine whether PMA also affected the processing of extracellular fluids. When the accumulation of horseradish peroxidase (HRP) was measured, the rate was found to be higher in PMA-treated cells from 5 min, the earliest time assayed, onward. The results suggest that the transformed type of cell in these cell lines showed a constitutive dilation and/or reorganization of some portion of the endosomal pathway.
机译：相对于正常的同行,改变了上皮细胞有一个独特的和可量化的三维形状。和数学方法是用来区分从两行,这些极端的细胞培养从大鼠肝脏和气管上皮细胞,分别。transformed-looking配置是暂时性的当暴露于佛波醇12-myristate 13-acetate(PMA)(普卢默赫克曼,[1990]Exp。细胞Res, 188:66 - 74)。是解剖生理过程参与的形状改变。已知PMA-stimulated在其他细胞调查。健壮的比正常,PMA刺激激怒活动5 h段。波动在哪里开始下降了5 h,然而,抑制被10 h。细胞两线采用了改变了形状莫能菌素配置当暴露2 h。当形状特征的子集改变最初与治疗改变了在变换,它被发现monensin-treated细胞模仿的特点转化细胞。然而,不像PMA的。可能源于激怒本身,而是可能是一个由波动过程。刺激细胞的特点转化细胞。能干扰核内体和吗内源性的处理内容,这是一个可能的行动。确定PMA也影响了细胞外液的处理。辣根过氧化物酶积累合测量,发现在高PMA-treated细胞从5分钟,最早的时候化验,向前。在这些细胞系转化类型的细胞显示本构扩张和/或重组endosomal的一部分途径。

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