Insulin-like growth factor I rescues SH-SY5Y human neuroblastoma cells from hyperosmotic induced programmed cell death.

Matthews CC; Feldman EL

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Insulin-like growth factor I rescues SH-SY5Y human neuroblastoma cells from hyperosmotic induced programmed cell death.

机译：胰岛素样生长因子I救援SH-SY5Y人类从高渗的诱导神经母细胞瘤细胞程序性细胞死亡。

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摘要

Insulin-like growth factor I (IGF-I) and the type I IGF receptor are widely distributed in developing and adult mammalian nervous systems. In vitro, IGF-I is a mitogen for primary neurons and also for cells from the SH-SY5Y human neuroblastoma cell line, a well-characterized model system of neuronal growth. In the current study, we examined the effects of osmotic stress on SH-SY5Y cell viability and the mechanism by which IGF-I serves as a neuronal osmoprotectant. Within 24 hr, exposure of SH-SY5Y cells to hyperosmotic serum-free media decreased (1) the number of viable cells, (2) the rate of 3H-thymidine incorporation, and (3) cell cycle progression. The inclusion of 10 nM IGF-I with hyperosmotic media prevented the loss of cell viability. The osmoprotective effects of IGF-I were inhibited by alpha-IR3, a blocking antibody of the type I IGF receptor. The observed loss of SH-SY5Y cell viability following hyperosmotic shock was due to an induction of programmed cell death as determined by flow cytometryand gel electrophoresis. Our results suggest that IGF-I can protect SH-SY5Y cells from hyperosmotic induced programmed cell death.

机译：胰岛素样生长因子I (IGF-I)和类型我IGF受体广泛分布于发展中国家和成年哺乳动物的神经系统。在体外,IGF-I是主要的有丝分裂原神经元和从SH-SY5Y人类细胞神经母细胞瘤细胞系,特征明显神经元增长的模型系统。研究中,我们研究了渗透压力的影响SH-SY5Y细胞生存能力和机制IGF-I作为神经元osmoprotectant。24小时内,SH-SY5Y细胞的接触无血清培养系统高渗的媒体(1)下降可行的细胞的数量,(2)的速度3 h-thymidine公司,(3)细胞周期进展。高渗的媒体阻止细胞的损失生存能力。被alpha-IR3抑制,抑制性抗体我IGF受体的类型。SH-SY5Y细胞生存能力高渗的休克是由于细胞程序性的感应死亡是由流cytometryand凝胶电泳。可以保护SH-SY5Y细胞高渗的吗诱导细胞程序性死亡。

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来源
《Journal of Cellular Physiology》 |1996年第2期|323-331|共9页
作者
Matthews CC; Feldman EL;
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作者单位

Department of Neurology, University of Michigan, Ann Arbor 48109, USA.;

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正文语种英语
中图分类细胞生物学;
关键词
Insulin-Like Growth Factor I; cell viability; Insulin-Like Growth Factor ReceptorIGF1 genetype I collagen receptorSH-SY5Y cell lineNeuronsNeurogenesisalpha beta interferon receptorOsmotic PressureApoptosis;

机译：胰岛素样生长因子I;细胞可行性;胰岛素样生长因子ReceptorIGF1信息我胶原蛋白receptorSH-SY5Y细胞lineNeuronsNeurogenesisalphaβ干扰素receptorOsmotic PressureApoptosis;

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Insulin-like growth factor I rescues SH-SY5Y human neuroblastoma cells from hyperosmotic induced programmed cell death.

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