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首页> 外文期刊>Journal of Cellular Physiology >Transforming growth factor beta 1 induces mitogenesis in fetal rat brown adipocytes.
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Transforming growth factor beta 1 induces mitogenesis in fetal rat brown adipocytes.

机译：转化生长因子β1诱发有丝分裂发生在胎鼠棕色脂肪细胞。

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摘要

The presence of transforming growth factor beta 1 (TGF-beta 1) for 24 or 48 h stimulated DNA synthesis, the percentage of cells in the S + G2/M phases of the cell cycle, and cell number, as compared to quiescent cells. The mitogenic capacity of TGF-beta 1 (1 pM) was similar to that shown by 10% fetal calf serum (FCS). TGF-beta 1 for 48 h increased by 5-fold the percentage of cells containing (3H)thymidine-labeled nuclei as compared to quiescent cels. In addition, single fetal brown adipocytes, showing their typical multilocular fat droplets phenotype, become positive for (3H)thymidine-labeled nuclei in response to TGF-beta 1. Moreover, TGF-beta 1 induced the mRNA expression of a complete set of proliferation-related genes, such as c-fos (30 min), c-myc and beta-actin (2 h), and H-ras, cdc2 kinase, and glucose 6-phosphate dehydrogenase (G6PD) at 4 and 8 h, as compared to quiescent cells. Concurrently, TGF-beta 1 for 12 h increased the protein content of proliferating cellular nuclear antigen (PCNA) by 6-fold and p21-ras by 2-fold. Although our results demonstrate that TGF-beta 1 induces the expression of very early genes related to cell proliferation, TGF-beta 1 could be acting either as a mitogen or as a survival factor in induce proliferation to fetal brown adipocytes.
机译：转化生长因子β1的存在(及1)24或48小时刺激DNA合成、S +细胞的比例G2 / M期细胞周期,细胞数量,相比静止细胞。能力及1(1点)是相似的显示10%胎牛血清(FCS)。48 h的比例增长了5倍包含(3 h) thymidine-labeled核细胞而塞尔静止。胎儿棕色脂肪细胞,显示他们的典型多腔的脂肪滴表型,成为(3 h) thymidine-labeled阳性细胞核反应及1。诱导的mRNA表达一套完整的扩散的基因,如c-fos (30分钟),原癌基因和beta-actin (2 h), h, cdc2葡萄糖激酶,6-phosphate脱氢酶(G6PD) 4和8 h,而静止细胞。增加蛋白质含量的激增细胞的核抗原(PCNA)的6倍p21-ras 2倍。证明及1诱发早期基因的表达与细胞有关增殖,及1可以代理作为一个有丝分裂原诱导或生存因素胎儿棕色脂肪细胞增殖。

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