...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >Phorbol 12-myristate 13-acetate enhances nm23 gene expression in murine melanocytes but not in syngeneic B16-BL6 melanoma variants.
【24h】

Phorbol 12-myristate 13-acetate enhances nm23 gene expression in murine melanocytes but not in syngeneic B16-BL6 melanoma variants.

机译：佛波醇12-myristate 13-acetate增强nm23基因在小鼠黑色素细胞而不是表达同系的B16-BL6黑色素瘤变体。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The nm23 gene has been described as a potential metastasis suppressor gene in certain rodent and human tumors. We previously demonstrated that tyrosine and phenylalanine restriction suppresses metastatic heterogeneity of B16-BL6 murine melanoma and selects for tumor variants with decreased metastatic potential. In this study, we investigated nm23 expression in the highly metastatic B16-BL6 (ND) melanoma, its nutritionally derived poorly metastatic (LT) variant, and the syngeneic non-tumorigenic Mel-ab melanocytes. No differences in nm23 expression were observed between ND and LT cells, and nm23 expression varied between different isolates. Previously, we showed that metastatic potential of 1-ND cells decreases and is not altered in 1-LT cells after prolonged in vitro cell passage; however, nm23 expression is equivalently increased by 2-fold. In 2-ND and 2-LT cells, expression of nm23 is not different at higher in vitro cell passage. Expression of nm23 decreased about 2-fold when phorbol 12-myristate 13-acetate (PMA) was removed from Mel-ab cells, which induces these cells to become quiescent. Although membrane-associated protein kinase C (PKC) activity decreased after prolonged PMA treatment in all cells, neither nm23 expression nor proliferation of ND and LT cells was affected by PMA. These data indicate that nm23 expression is related to proliferative activity rather than to the suppression of metastatic potential.
机译：nm23基因已被描述为一个潜在的在某些啮齿动物和转移抑制基因人类肿瘤。抑制酪氨酸和苯丙氨酸限制转移性异质性B16-BL6小鼠黑色素瘤和肿瘤变体的选择减少转移的潜力。研究nm23在高度表达黑色素瘤转移性B16-BL6 (ND),它的营养派生差转移(LT)变体,同系的非致瘤性Mel-ab黑色素细胞。ND和肝细胞之间的观察,和nm23吗表达不同分离株之间的不同。以前,我们表明,转移的潜力1-ND细胞的减少并没有改变1细胞经过长时间的体外细胞通道;然而,nm23表达式是等价的增加了2倍。nm23的表达不是不同的更高体外细胞的通道。大约2倍当佛波醇12-myristate 13-acetate(PMA)被撤Mel-ab细胞,这种细胞诱导这些细胞成为静止。膜相关蛋白激酶C (PKC)活动长期PMA治疗后下降在所有细胞中,无论是nm23表达式ND和肝细胞增殖的影响PMA。而不是增殖相关活动的抑制转移的潜力。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号