Minocycline impairment of both osteoid tissue removal and osteoclastic resorption in a synchronized model of remodeling in the rat.

Klapisz Wolikow M; Saffar JL

摘要

In addition to their antibacterial effects, tetracyclines may inhibit interstitial collagenase activity and bone resorption. These properties were assessed morphometrically using minocycline (25 and 50 mg/kg/day given by the IM route) in a rat model of synchronized remodeling in which osteoclastic resorption peaks 4 days after the activating event (the extractions of the upper molars) along the antagonist mandibular cortex, a zone undergoing physiologically active formation. During the first 2 days of activation, minocycline at the two doses impaired very significantly the disorganization of both the osteoid seam and the layer of osteoblasts, a prerequisite to give osteoclasts access to the mineralized bone surface. The number of readily identifiable osteoblasts decreased slightly during this period, suggesting that minocycline prevented their transformation into lining cells. Their synthetic activity, as estimated by the size of the cells and their nucleus, appeared relatively preserved too, mostly with the higher dose. AT the peak of osteoclasia, the bone surfaces undergoing remodeling were significantly decreased in the minocycline-treated groups. The resorption surface was reduced (P < 0.0003) as well as the number of osteoclasts (P < 0.0007), which were also significantly smaller. Their resorbing activity was dramatically affected as well: they excavated lacunae whose area was significantly reduced by over 70%. In addition, formation was still a prominent activity in the treated animals. These data are compatible with the inhibition at the early stages of activation of an osteoblast-secreted collagenase whose action may be the elimination of the osteoid seam. The inhibition of an osteoclast collagenase and/or of a bone matrix bound-collagenase may be responsible for the reduction in lacunar size. A direct effect of minocycline on osteoclast resorptive activity may also participate in the low resorption profile, as tetracyclines are known to interfere with the intracellular [Ca2+].

机译：除了他们的抗菌效果,四环素可能抑制间隙胶原酶活性和骨吸收。属性评估morphometrically使用二甲胺四环素(25 - 50毫克/公斤/天的IM路线)在大鼠模型中重构同步监测吸收峰4天激活事件(拔掉后沿着拮抗剂下颌上臼齿)皮层区域进行生理活动形成。二甲胺四环素在两剂很受损明显的混乱seam和成骨细胞层,类骨质先决条件给破骨细胞访问矿化骨表面。可识别的成骨细胞略有下降在此期间,这表明二甲胺四环素阻止他们转换成衬里细胞。他们的合成活动,如估计的细胞和细胞核的大小,出现了相对保存,主要与更高剂量。表面进行改造是显著的减少minocycline-treated组。吸收表面减少(P < 0.0003)破骨细胞的数量(P < 0.0007),这也小得多。突起活动显著影响:他们发掘缺损的区域大大减少了超过70%。形成仍然是一个突出的活动对待动物。抑制活化的早期阶段的osteoblast-secreted胶原酶的行动可能会消除类骨质seam。和/或骨基质的bound-collagenase负责降低腔隙的大小。直接二甲胺四环素对破骨细胞的影响再吸收的活动也可能参与低吸收剖面、四环素已知干扰细胞内[Ca2 +]。

Minocycline impairment of both osteoid tissue removal and osteoclastic resorption in a synchronized model of remodeling in the rat.

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