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首页> 外文期刊>Journal of Cellular Physiology >Heat shock induction of apoptosis in promastigotes of the unicellular organism Leishmania (Leishmania) amazonensis.
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Heat shock induction of apoptosis in promastigotes of the unicellular organism Leishmania (Leishmania) amazonensis.

机译：在promastigotes热休克诱导细胞凋亡的单细胞有机体利什曼虫(利什曼原虫)amazonensis。

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Apoptosis and/or programmed cell death have been described in examples ranging from fungi to man as gene-regulated processes with roles in cell and tissue physiopathology. These processes require the operation of an intercellular communicating network able to deliver alternative signals for cells with different fates and is thus considered a prerogative of multicellular organisms. Promastigotes from Leishmania (Leishmania) amazonensis, when shifted from their optimal in vitro growth temperature (22 degrees C) to the temperature of the mammalian host (37 degrees C), die by a calcium-modulated mechanism. More parasites die in the presence of this ion than in its absence, as detected by a colorimetric assay based on the activity of mitochondrial and cytoplasmic dehydrogenases which measures cell death, independently of the process by which it occurs. A heat shock, unable to induce detectable parasite death (34 degrees C for 1 h), is able to significantly raise the concentration of intracellular free calcium in these cells. Heat-shocked parasites present ultrastructural and molecular features characteristic of cells dying by apoptosis. Morphological changes, observed only in the presence of calcium, are mainly nuclear. Cytoplasmic organelles are preserved. Heat shock is also able to induce DNA cleavage into an oligonucleosomal ladder detected in agarose gels by ethidium bromide staining and autoradiography of [alpha 32P]ddATP-labeled fragments. These results indicate that death by apoptosis is not exclusive of multicellular organisms.
机译：细胞凋亡或程序性细胞死亡中描述的例子从真菌是由基因和角色在细胞过程和组织生理病理学。需要一个细胞间的操作通信网络能够提供选择信号与不同的命运,并且细胞因此被认为是多细胞的特权生物。(利什曼虫)amazonensis,当从他们的最佳的体外生长温度(22度C)哺乳动物宿主的温度(37度),死于一个calcium-modulated机制。有寄生虫的死在这个离子的存在比在其缺席,如检测到一个基于活动的比色测定线粒体和细胞质脱氢酶衡量细胞死亡的独立它发生的过程。诱导检测疟原虫死亡(34摄氏度1 h),能够显著提高细胞内自由钙的浓度这些细胞。超微结构和分子特性细胞死亡的凋亡特征。形态变化,观察到的钙的存在,主要是核。胞质细胞器。也能够诱导DNA分裂成一个oligonucleosomal梯子在琼脂糖凝胶中发现溴化乙锭染色法和放射自显影法(α32 p) ddATP-labeled碎片。结果表明,死亡的细胞凋亡独家的多细胞生物。

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