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首页> 外文期刊>Journal of Cellular Physiology >cGMP accumulation and gene expression of soluble guanylate cyclase in human vascular tissue.
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cGMP accumulation and gene expression of soluble guanylate cyclase in human vascular tissue.

机译：cGMP积累和基因表达的可溶性鸟苷酸环化酶在人类血管组织。

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摘要

Gene expression of soluble guanylate cyclase (sGC) and cGMP accumulation in response to sodium nitroprusside (SNP) were studied in cultured human vascular cells and freshly harvested vascular tissue. As revealed by reverse transcriptase-polymerase chain reaction, cultured smooth muscle and endothelial cells, as well as freshly isolated human vascular tissue, express mRNA for the alpha 3 and beta 3 subunits but not for the alpha 2 and beta 3 subunits is evident even in the absence of increased cGMP accumulation in response to SNP. cGMP accumulation in human cells cultured from different vascular beds typically increased two- to five-fold (maximum of 11.4-fold) over baseline following stimulation with 100 microM SNP. Bovine, murine, canine, and avian vascular smooth muscle cells accumulated similar or lower amounts of cGMP than human cells, whereas porcine, rat, and rabbit smooth muscle cells accumulated greater amounts of cGMP. In freshly harvested human vessels, cGMP accumulation in response to SNP wasfound to increase fifteen-fold over baseline. In contrast to the SNP-induced cGMP accumulation, cGMP levels in response to particulate guanylate cyclase activator atriopeptin II were equal to or greater in cultured human cells than in fresh human vascular tissue. We conclude that human vascular cells (fresh and cultured) express the mRNA for both a large (alpha 3) and a small (beta 3) sGC subunit and that fresh human cells are more sensitive to SNP stimulation.
机译：基因表达的可溶性鸟苷酸环化酶(国网公司)和cGMP积累应对钠硝普酸(SNP)在培养进行了研究人体血管细胞和新收获维管组织。transcriptase-polymerase连锁反应,讲究的平滑肌和血管内皮细胞,以及新孤立的人类的维管组织,表达信使rna的α和β3单元而不是的α2β3单元是显而易见的即使没有cGMP的增加积累对SNP的回应。积累在人体细胞培养不同的血管床通常增加了两个-最多5倍(11.4倍)基线后与100年刺激microM SNP。牛,鼠、犬类和鸟类血管平滑肌肉细胞积累类似或更低cGMP的人类细胞,而猪、老鼠,和兔平滑肌细胞积累cGMP的更多。人类的船只,cGMP积累在回应SNP吧增加大蒜基线。积累,cGMP水平以应对微粒鸟苷酸环化酶激活剂atriopeptin II是等于或大于比新鲜的人类血管培养的人类细胞组织。(新鲜和培养)mRNA的表达大(α3)和小(β3)国网公司亚基新鲜人体细胞更敏感SNP的刺激。

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