...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >Basic fibroblast growth factor (FGF-2) protects rat cochlear hair cells in organotypical culture from aminoglycoside injury.
【24h】

Basic fibroblast growth factor (FGF-2) protects rat cochlear hair cells in organotypical culture from aminoglycoside injury.

机译：碱性纤维母细胞生长因子(FGF-2)保护大鼠耳蜗毛细胞organotypical文化从氨基糖苷类伤害。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Given the evidence that basic fibroblast growth factor (FGF-2) can protect neural and retinal cells from degeneration, we evaluated the potential of this growth factor to protect sensory cells in the inner ear. When sensory cells of the organ of Corti are exposed to aminoglycoside antibiotics such as neomycin either in vivo or in vitro, significant ototoxicity is observed. The in vitro cytotoxic effects of neomycin are dose and time dependent. In neonatal rat organ of Corti cultures, complete inner and outer hair cell destruction is observed at high (mM) concentrations of neomycin while inner hair cell survival and severely damaged outer hair cells are noted at moderate (microM) concentrations, with a maximal effect observed after 2 days of culture. Approximately 50% of cochlear outer hair cells are lost at a dose of 35 microM neomycin, and most surviving cells show disorganized stereocilia. Inner hair cells show primarily disorganization of their stereocilia. A significant protective effect is observed when the organ of Corti is pre-treated with FGF-2 (500 ng/ml) for 48 hours, and then FGF-2 is included with neomycin in the culture medium. A greater extent of outer hair cell survival and a significant decrease in stereociliary damage are noted with FGF-2. However, disorganization of inner hair cell stereocilia is unaffected by FGF-2. The protective effect of FGF-2 is specific, since interleukin-1B, nerve growth factor, tumor necrosis factor, and epidermal growth factor are ineffective, while retinoic acid and transforming growth factor alpha show only a moderate protective effect. These results confirm the potential of molecules like FGF-2 for preventing cell death due to a variety of causes.
机译：考虑到基本的纤维母细胞生长的证据因子(FGF-2)可以保护神经和视网膜细胞变性,我们评估了这种增长潜力因素来保护内耳感觉细胞。柯蒂氏器官细胞接触氨基苷类抗生素,如新霉素体内或体外,意义重大耳毒性观察。新霉素是时间和剂量依赖的影响。在新生鼠螺旋器文化中,完成内外毛细胞破坏在高(mM)的浓度新霉素内毛细胞生存和严重受损外毛细胞在温和的指出(microM)浓度,观察到的最大影响经过2天的文化。耳蜗外毛细胞丢失的剂量35 microM新霉素,大多数活细胞杂乱无章的静纤毛。主要是无序的静纤毛。时观察到显著的保护作用螺旋器与FGF-2预处理(500ng / ml) 48小时,然后FGF-2包括在内新霉素的培养基。外毛细胞生存的程度显著降低stereociliary损害与FGF-2指出。内毛细胞静纤毛是影响FGF-2。具体的,因为interleukin-1B,神经生长因子、肿瘤坏死因子、表皮生长因子是无效的,而维生素a酸和转化生长因子α只是一个温和的保护作用。像FGF-2确认潜在的分子防止细胞死亡由于各种各样的原因。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号