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首页> 外文期刊>Journal of Cellular Physiology >Effects of osteogenic protein-1 (OP-1, BMP-7) on bone matrix protein expression by fetal rat calvarial cells are differentiation stage specific.
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Effects of osteogenic protein-1 (OP-1, BMP-7) on bone matrix protein expression by fetal rat calvarial cells are differentiation stage specific.

机译：影响成骨蛋白1 (OP-1 BMP-7)胎鼠骨基质蛋白表达颅顶的细胞分化阶段具体。

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Bone morphogenetic proteins (BMPs) are a group of cytokines that are characterized by their ability to stimulate osteoblast differentiation and bone formation. However, the influence of BMPs on osteoblastic cells at different stages of differentiation is not known. Since bone matrix proteins are differentially regulated during bone formation we have studied the effects of recombinant human osteogenic protein-1 (rhOP-1; BMP-7) on the expression of these proteins by fetal rat calvarial cells (FRCCs) at discrete stages of osteoblast differentiation. Continuous administration of rhOP-1 to FRCCs, beginning at confluence (day 7), produced a dose-dependent increase in the number, size and mineralization of bone-like nodules formed in the presence of vitamin C and beta-glycerophosphate. Within 9 h of administration, rhOP-1 stimulated a 3-fold increase in OPN mRNA which was reflected in a comparable increase in the low phosphorylated, 55 kDa form of osteopontin. In contrast, changes in type 1 collagen, alkalinephosphatase and bone sialoprotein mRNAs followed the differentiation of preosteoblastic cells, and were increased 2-, 4- and 5-fold, respectively, after 8 days (day 15). When administered at intermediate stages of osteoblast differentiation (days 12, 15 and 18) BSP remained refractory to rhOP-1 whereas the ALP was increased almost 2-fold, independent of the constitutive levels of mRNA expression. To determine the effects on osteoblasts, FRCCs were first grown to the bone nodule-forming stage (day 21) before rhOP-1 was administered. Only modest, transient increases in the expression of ALP and OPN mRNAs were evident whereas OC expression was increased more than 3-fold. In contrast, collagen type 1 and BSP mRNA levels were not changed significantly. These results suggest that rhOP-1 increases bone formation by promoting osteoblastic differentiation, as indicated by the increased number of bone forming colonies and by increasing the number of osteoblastic cells in the colonies, but not by increasing matrix production by individual osteoblasts. It is also evident that the regulation of bone matrix proteins by rhOP-1 is dependent upon the differentiated state of the cell.
机译：骨形成蛋白(bmp)是一组的细胞因子的特点是他们的能力刺激成骨细胞分化和骨形成。在不同阶段的成骨细胞的细胞分化尚不清楚。在骨蛋白质不同监管形成我们的影响进行了研究重组人类成骨蛋白1 (rhOP-1;BMP-7)这些蛋白的表达胎鼠颅顶的细胞(FRCCs)离散成骨细胞分化阶段。政府rhOP-1 FRCCs,开始融合(7天),产生剂量依赖性增加数量,大小和矿化骨突的结节的形成维生素C和beta-glycerophosphate。政府,rhOP-1刺激三倍增加OPN mRNA反映在一个增加可比性低磷酸化,55骨桥蛋白kDa的形式。1型胶原,alkalinephosphatase和骨头唾液蛋白mrna的分化preosteoblastic细胞,增加2 -4 - 5倍,分别在8天(一天15)。成骨细胞分化(天12、15和18)BSP仍然耐火rhOP-1而高山是增加了近2倍,独立的本构的信使rna表达水平。确定对成骨细胞的影响,FRCCs第一次种植骨nodule-forming阶段(一天21)在rhOP-1实施之前。在高山的表达和瞬态增加OPN mrna是明显而OC表达式增长了三倍多。1型和BSP mRNA水平并没有改变显著。增加通过促进骨形成成骨细胞的分化,如所示数量的增加骨形成的殖民地增加成骨细胞的细胞的数量殖民地,但不是通过增加矩阵生产单独的成骨细胞。明显,骨基质的监管由rhOP-1依赖于蛋白质细胞的分化状态。

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