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首页> 外文期刊>Journal of Cellular Physiology >Platelet-derived growth factor and inflammatory cytokines have differential effects on the expression of integrins alpha 1 beta 1 and alpha 5 beta 1 by human dermal fibroblasts in vitro.
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Platelet-derived growth factor and inflammatory cytokines have differential effects on the expression of integrins alpha 1 beta 1 and alpha 5 beta 1 by human dermal fibroblasts in vitro.

机译：血小板源生长因子和炎症细胞因子有不同的影响整合素α1β1和α的表达5β1由人类皮肤成纤维细胞在体外。

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摘要

Dermal fibroblasts are essential for the repair of cutaneous wounds. Fibroblasts presumably use cell surface receptors of the integrin family during migration into a wound from the adjacent uninjured tissue and for the subsequent matrix repairs. We have investigated the possible roles of platelet-derived growth factor and inflammatory cytokines in the regulation of integrin expression on wound fibroblasts using a porcine cutaneous wound model and cultured human cells. Tissue specimens collected from 4-day pig wounds were stained with antibodies specific for the alpha 1 and alpha 5 integrin subunits. Staining for alpha 1 was markedly decreased on fibroblasts adjacent to the wound and in the granulation tissue, while staining for alpha 5 was clearly enhanced in both locations. Normal adult human dermal fibroblasts in culture express the integrins alpha 1 beta 1, a collagen receptor, and alpha 5 beta 1 a fibronectin receptor. Quantitative flow cytometry was used to measure cell surface integrin expressionafter treatment with platelet-derived growth factor (PDGF)-AA, PDGF-AB, or PDGF BB. Each isoform of PDGF produced a significant decrease in the level of alpha 1 present on the cell surface and an increase in the level of alpha 5. Furthermore, PDGF-BB produced a corresponding decrease in alpha 1 mRNA and an increase in alpha 5 mRNA. In contrast, treatment with three inflammatory cytokines, IL-1 beta, TNF-alpha, and IFN-gamma, produced clear increases in the levels of alpha 1 and alpha 5 present on the cell surface. Our observations suggest that the differential effects of PDGF and inflammatory cytokines may be part of the mechanism regulating the expression of alpha 1 and alpha 5 integrins by dermal fibroblasts during wound repair.
机译：真皮成纤维细胞的修复至关重要皮肤的伤口。表面受体整合素家族的期间从相邻迁移到伤口受伤组织和随后的矩阵维修。血小板源生长因子和炎性细胞因子的调节在伤口成纤维细胞整合素表达使用猪皮肤伤口模型和人工培养的细胞。伤口沾染了特定的抗体5整合素α1和α亚基。染色α1明显减少成纤维细胞相邻伤口的肉芽组织,而染色α5在两个地方很明显增强。成人真皮成纤维细胞在文化表达整合素α1β1,胶原蛋白纤连蛋白受体,α5β1受体。测量细胞表面整合素expressionafter与血小板源生长因子治疗(PDGF) aa、PDGF-AB或PDGF BB。PDGF生产水平显著下降α1呈现在细胞表面和一个α5的增长水平。PDGF-BB产生相应的减少α1 mRNA和增加α5 mRNA。相反,使用三种炎症进行治疗β细胞因子,il - 1、tnf和IFN-gamma,产生明显的增加α1的水平α5呈现在细胞表面。观察结果表明微分PDGF和炎性细胞因子的影响机制调节表达式的一部分α1和α5整合蛋白的真皮成纤维细胞在创伤修复。

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