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首页> 外文期刊>Journal of Cellular Physiology >Differential growth factor responses of epithelial cell cultures derived from normal human prostate, benign prostatic hyperplasia, and primary prostate carcinoma.
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Differential growth factor responses of epithelial cell cultures derived from normal human prostate, benign prostatic hyperplasia, and primary prostate carcinoma.

机译：微分的上皮生长因子响应细胞培养来自正常的人类前列腺,良性前列腺增生和主前列腺癌。

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Because of a lack of information of the optimum nutritional requirements, epithelial cells derived from normal human prostate and prostate tumors have been difficult to propagate in vitro, which hinders research in prostate carcinogenesis. In an effort to establish optimum nutritional conditions and differences in growth characteristics of normal human prostate (NP), benign prostatic hyperplasia (BPH), and prostatic carcinoma (PCA), we have compared the effects of several growth factors on cell proliferation and elucidated growth properties of low passage epithelial cells derived from NP, BPH, and PCA of an African-American patient. Primary and low passage cultures were propagated in serum-free keratinocyte basal medium (KBM) supplemented with insulin (5 micrograms/ml), hydrocortisone (0.5 microgram/ml), epidermal growth factor (EGF, 10 ng/ml), bovine pituitary extract (BPE; 50 micrograms/ml), cholera toxin (10 ng/ml), and antibiotics. Almost all NP, BPH, and PCA cells were positive for cytokeratins and prostate-specific antigen (PSA). The NP, BPH, and PCA cells were essentially diploid and lacked mutations in c-K-ras and c-Ha-ras oncogenes, and p53 tumor suppressor gene. However, they exhibited progressively accelerating growth parameters. The population doubling times of NP, BPH and PCA were 51 hr, 37 hr, and 29 hr, respectively; their saturation densities were 2.9 x 10(4)/cm2, 3.3 x 10(4)/cm2, and 7.2 x 10(4)/cm2, respectively. The NP and BPH cells required all of the growth factors in the medium, as deletion of any one of the above factors strongly inhibited their growth. The PCA cells, however, were independent of EGF and hydrocortisone. PC-3, an established human prostate cancer cell line, was independent of the growth factors tested. Fetal bovine serum (FBS) inhibited the growth of NP, BPH and PCA cells. In contrast, FBS stimulated the growth of the PC-3 cells in a concentration-dependent manner. These results indicate that in the absence of any apparent karyotype alterations and mutations in c-K-ras, c-Ha-ras and p53 genes, epithelial cells derived from NP, BPH, and PCA exhibit significant differences in their growth properties and responses to growth factors. These variations may represent early changes involved in prostate cancer, while gene mutations and cytogenetic alterations occur in advanced and/or metastatic tumors.
机译：由于缺乏信息的最优营养需求,上皮细胞来自正常的人类前列腺癌和前列腺癌体外肿瘤难以传播,阻碍了研究前列腺癌吗致癌作用。营养条件和发展的差异正常的人类前列腺癌的特点(NP),良性前列腺增生(BPH)和前列腺癌(PCA),而所带来的影响一些在细胞增殖和生长因子阐明增长低段的属性上皮细胞来源于NP、前列腺肥大和主成分分析病人一个非洲裔美国人。通过文化传播在无血清角化细胞基础培养基(KBM)补充胰岛素(5微克/毫升)、氢化可的松(0.5微克/毫升)、表皮生长因子(EGF), 10ng / ml),牛脑垂体提取物(BPE;微克/毫升),霍乱毒素(10 ng / ml)抗生素。细胞角蛋白阳性,前列腺特异性抗原(PSA)。PCA细胞本质上都是二倍体和缺乏突变c-K-ras c-Ha-ras致癌基因,p53肿瘤抑制基因。表现出逐渐加速增长参数。37岁的良性前列腺增生和PCA 51人力资源人力资源,和29日人力资源,分别;x 10 (4) / cm2, 3.3 x 10 (4) / cm2,和7.2 x分别为10 (4)/ cm2。要求所有的生长因子中,删除任何上述因素之一强烈抑制经济增长。然而,EGF和独立的氢化可的松。前列腺癌细胞系,是独立的生长因子测试。抑制NP的增长,前列腺肥大和PCA细胞。相反,的边后卫曲泽刺激增长细胞浓度的方式。结果表明,在缺乏任何明显的核型改变和突变c-K-ras c-Ha-ras和p53基因,上皮细胞来自NP、前列腺肥大和PCA展览意义重大差异属性和增长对生长因子的反应。代表参与前列腺癌早期变化癌症,而基因突变和细胞遗传学改变发生在发达国家和/或转移肿瘤。

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