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首页> 外文期刊>Journal of Cellular Physiology >Analysis of EPC-1 growth state-dependent expression, specificity, and conservation of related sequences.
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Analysis of EPC-1 growth state-dependent expression, specificity, and conservation of related sequences.

机译：分析EPC-1增长依赖政府表情,特异性和保护相关的序列。

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摘要

The transcript for EPC-1 (early population doubling level (PDL) cDNA-1) is induced under conditions of growth arrest due to density-dependent contact inhibition and/or serum deprivation in early-passage but not in senescent WI-38 fibroblasts. We have characterized the EPC-1 transcript with respect to its cell-cycle regulation, tissue specificity, and interspecies conservation of related genomic sequences. In low density, quiescent (serum-deprived), early-passage fibroblasts that are stimulated to proliferate with fresh serum, steady-state EPC-1 transcript levels are steadily reduced until they reach a basal level approximately 24 h after stimulation. However, when early-passage fibroblasts are made quiescent by both serum deprivation and density-dependent contact inhibition and then stimulated with serum, steady-state EPC-1 transcript levels remain relatively constant throughout a 36 h period following serum stimulation. Senescent WI-38 cells (> 95% life span completed) do not express EPC-1 under theseconditions. We show that differences in the regulation of EPC-1 transcript levels in early-passage cells are due to differences in growth state rather than changes in cell density or contact. We also show that expression of the EPC-1 transcript is limited to specific cell types and that related genomic sequences are found in all mammalian species examined as well as in the chicken.
机译：EPC-1成绩单(早期的人口翻倍级别(PDL) cDNA-1)诱导下增长逮捕的条件密度制约的接触抑制和/或血清剥夺early-passage但不衰老WI-38成纤维细胞。EPC-1记录对其细胞循环规定,组织特异性和种间保护相关的基因组序列。密度、静(serum-deprived),early-passage刺激成纤维细胞增殖与新鲜血清,稳态EPC-1转录水平稳步减少,直到他们达到基础水平大约24 h后刺激。成纤维细胞是由静止的血清剥夺和密度制约的接触然后用血清刺激抑制,稳态EPC-1转录水平仍然存在在36小时内相对稳定后血清刺激。电池寿命(> 95%)完成不表达EPC-1这些疾病。监管的差异EPC-1成绩单是由于水平early-passage细胞不同的增长状态,而不是改变在细胞密度或接触。的表达EPC-1成绩单是有限的特定的细胞类型和相关的基因序列是所有哺乳动物物种中发现的检查以及鸡肉。

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