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首页> 外文期刊>Journal of Cellular Physiology >Zinc inhibits turnover of labile mRNAs in intact cells.
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Zinc inhibits turnover of labile mRNAs in intact cells.

机译：锌抑制营业额不稳定mrna的完好无损细胞。

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摘要

For immediate early genes such as the c-fos proto-oncogene, mRNA breakdown is very rapid and is largely responsible for the transient nature of mRNA accumulation after transcription is stimulated. We found that in several types of cultured cells and in mice, Zn++ caused marked accumulation of c-fos mRNA and that of another labile mRNA, that encoding the tristetraprolin (TTP) protein. Exposure of TK-L cells to 100 microM ZnSO4 caused an increase of c-fos and TTP mRNA levels within 1 h that reached peak levels in 4-8 h and remained constant to 12 h. Increases in fos protein accumulation were also noted. When the cells were exposed to Zn++ for 4 h and then exposed to actinomycin D, both c-fos and TTP mRNA levels remained constant for up to 10 h, indicating that Zn++ was preventing the breakdown of both c-fos and TTP mRNA. Also, 100 microM ZnSO4 inhibited protein synthesis in TK-L cells, suggesting that the effect on mRNA accumulation could have been an indirect effect resulting from inhibited protein synthesis. Zn++ was unable to inhibit the breakdown of TTP and c-fos mRNA in vitro; however, extracts from cells exposed to Zn++ were less able to cause the breakdown of TTP and c-fos mRNAs than were extracts from control cells, again suggesting that Zn++ indirectly affects mRNA stability through inhibition of protein synthesis. These studies suggest that in addition to their effects on gene transcription, Zn++ and other divalent cations may regulate gene expression by affecting mRNA stability.
机译：立即早期基因如c-fos原癌基因,信使rna分解非常迅速主要是负责瞬态性质信使rna转录后积累刺激。培养细胞和小鼠、锌+ +标志引起的积累c-fos mRNA和另一个不稳定的信使rna, tristetraprolin编码(TTP)的蛋白质。microM ZnSO4 c-fos和TTP的增加引起的信使rna水平在1 h达到峰值水平在4 - 8 h和保持不变到12 h。增加”丛书蛋白质积累也指出。细胞暴露在锌+ + 4 h,然后暴露于放线菌素D, c-fos和TTP mRNA10 h水平保持不变,表明锌+ +是防止崩溃c-fos和TTP mRNA。ZnSO4 TK-L细胞抑制蛋白质合成,这表明对信使rna的积累的影响可能是造成间接影响呢抑制蛋白质合成。抑制TTP的分解和c-fos信使rna体外;锌+ +不太能够引起TTP的崩溃从控制和c-fos mrna比提取细胞,又间接地表明锌+ +通过抑制影响mRNA稳定蛋白质合成。其对基因转录的影响之外,锌+ +和其他二价阳离子可以调节基因通过影响mRNA表达稳定。

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