Laminin decreases PRL and IGFBP-1 expression during in vitro decidualization of human endometrial stromal cells.

Brar AK; Frank GR; Richards RGMeyer AJKessler CACedars MIKlein DJHandwerger S

摘要

The expression of laminin, a major constituent of endometrial cell basement membranes, is increased during differentiation of human endometrial stromal cells (decidualization). To determine whether laminin plays a role in decidualization, we studied the effects of laminin substrate on the synthesis and release of prolactin (PRL) and insulin-like growth factor binding protein-1 (IGFBP-1), two major secretory proteins of decidualized stromal cells. Endometrial stromal cells were plated on laminin as well as several other extracellular matrix (ECM) proteins (types 1 and IV collagen or fibronectin) and on plastic, and cultured in media containing medroxyprogesterone acetate (MPA) and estradiol. Cells cultured on plastic or ECM proteins displayed similar morphological changes indicative of decidualization. However, the release of PRL and IGFBP-1 from cells cultured on plastic and ECM proteins (types 1 and IV collagen and fibronection) was approximately 2.1-fold and 2.8-fold greater respectively, than from cells cultured on laminin. The decrease in PRL and IGFBP-1 expression in cells cultured on laminin was not due to differences in initial cell attachment efficiency or final DNA content. In addition, laminin had no effect on the content of laminin protein or fibronectin mRNA levels, indicating that the effects of laminin on PRL and IGFBP-1 were specific. PGE2 stimulated the release of PRL and IGFBP-1 from cells cultured on laminin to levels comparable to those from cells cultured on plastic or other ECM proteins. This indicates that the decrease in PRL and IGFBP-1 release by laminin was not due to a generalized unresponsiveness. In contrast to the effects of laminin during decidualization, PRL expression was not altered by laminin in terminally differentiated decidual cells isolated at term. Our results support a role for laminin in selectively regulating PRL and IGFBP-1 gene expression during in vitro decidualization of human endometrial stromal cells.

机译：层粘连蛋白的表达,一个主要的组成部分子宫内膜细胞基底膜,增加在人类子宫内膜的分化基质细胞(decidualization)。层粘连蛋白是否在decidualization扮演了一个角色,我们研究了层粘连蛋白底物的影响的合成和释放催乳素(PRL)和胰岛素样生长因子结合蛋白1(IGFBP-1),两个主要分泌的蛋白质decidualized基质细胞。层粘连蛋白以及几个细胞被镀其他细胞外基质(ECM)蛋白(类型1和4胶原蛋白或纤连蛋白)和塑料,并在媒体包含培养甲孕酮(MPA)和雌二醇。细胞培养在塑料或ECM蛋白质显示类似的形态变化decidualization的象征。释放的PRL和IGFBP-1细胞培养塑料和ECM(类型1和静脉注射胶原蛋白的蛋白质和fibronection)大约是2.1倍2.8倍大的分别,比从细胞培养在层粘连蛋白。IGFBP-1表达层粘连蛋白在细胞培养不是由于初始细胞的差异附件效率或最终的DNA含量。此外,层粘连蛋白没有影响的内容层粘连蛋白蛋白质或纤连蛋白mRNA水平,这表明在PRL和层粘连蛋白的影响IGFBP-1是特定的。释放的PRL和IGFBP-1细胞培养层粘连蛋白从细胞水平与这些培养在塑料或其他ECM蛋白质。表明,PRL和IGFBP-1下降释放层粘连蛋白并不是由于广义反应迟钝。层粘连蛋白在decidualization, PRL表达不是在晚期被层粘连蛋白改变吗差异化的蜕膜细胞孤立的词。我们的研究结果支持层粘连蛋白的作用有选择地调节PRL和IGFBP-1基因在体外decidualization表达式人类子宫内膜基质细胞。

Laminin decreases PRL and IGFBP-1 expression during in vitro decidualization of human endometrial stromal cells.

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