...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >Characterization of an electrogenic sodium/glucose cotransporter in a human colon epithelial cell line.
【24h】

Characterization of an electrogenic sodium/glucose cotransporter in a human colon epithelial cell line.

机译：描述一个电致钠/葡萄糖转运蛋白在人类结肠上皮细胞线。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study, we have characterized the Na/glucose transporter in polarized monolayers formed by the clonal human colon carcinoma cell line HT-29-D4. Isotopic tracer flux measurements show that differentiated HT-29-D4 cells possess a sodium-dependent alpha-methyl-D-glucopyranoside (AMG) uptake that is competed for by increasing concentrations of D-glucose, D-galactose, and phlorizin. This transport is exclusively localized on the apical side of the epithelium. Kinetic data demonstrate the existence of a single Michaelian sodium-dependent AMG transporter with a Km of 1.2 +/- 0.12 mM and a Vmax of 3.24 +/- 0.25 nmol/mg of protein per min. Hill analysis reveals a coefficient of 1.9 +/- 0.03, consistent with at least two sodium ions involved in AMG transport. Interestingly, the cotransporter function is not modulated by glucose in the culture medium. Transepithelial electrical parameter measurements show that the transepithelial potential difference (Vt) is glucose dependent and phlorizin sensitive. Antibodies directed against a peptide of the rabbit intestinal glucose cotransporter (Ser402-Lys420) recognize, in western blot experiments, the characteristic bands of the cotransporter on a crude membrane preparation of differentiated HT-29-D4 cells and react strongly with the apical domain of the monolayer in immunofluorescence experiments. We conclude that HT-29-D4 cells express the sodium/glucose cotransporter SGLT1 at their apical membrane and that this transporter generates the basal transepithelial potential difference.
机译：在这项研究中,我们为特征Na /葡萄糖转运体在偏振膜克隆人类结肠癌癌形成的细胞行HT-29-D4。表明,分化HT-29-D4细胞拥有sodium-dependent alpha-methyl-D-glucopyranoside(AMG)吸收,是通过增加竞争浓度的葡萄糖、D-galactose和根皮甙。本地化在上皮细胞的顶端上。动力学数据展示的存在单一Michaelian sodium-dependent AMG运输机的公里和1.2 + / - 0.12毫米Vmax 3.24 + / - 0.25 nmol /每分钟毫克的蛋白质。希尔的分析揭示了一个系数是1.9 + / -0.03,符合至少两个钠离子参与AMG运输。转运蛋白功能不调葡萄糖的培养基。电参数测量显示transepithelial电位差(Vt)葡萄糖和根皮甙敏感的依赖。针对的肽的抗体兔子肠道葡萄糖转运蛋白(Ser402-Lys420)承认,在免疫印迹实验中,乐队的特征在原油转运蛋白膜的制备分化HT-29-D4细胞和强烈反应单层的顶端域免疫荧光实验。HT-29-D4细胞表达/钠葡萄糖转运蛋白SGLT1顶端膜这个运输产生基底transepithelial电位差。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号