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首页> 外文期刊>Journal of Cellular Physiology >Hepatocyte growth factor is involved in formation of osteoclast-like cells mediated by clonal stromal cells (MC3T3-G2/PA6).
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Hepatocyte growth factor is involved in formation of osteoclast-like cells mediated by clonal stromal cells (MC3T3-G2/PA6).

机译：肝细胞生长因子参与形成osteoclast-like细胞介导的克隆基质细胞(MC3T3-G2 /尼龙6)。

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摘要

Osteoclast formation from hemopoietic precursors has been shown to require the support of stromal cells in bone tissue. In this study, we demonstrated that hepatocyte growth factor (HGF) is one of the stromal cell-derived molecules responsible for osteoclast-like cell formation. For our experiments, we used a coculture system for osteoclastic cell formation and activation in which hemopoietic blast cells are cocultured with calvaria-derived stromal MC3T3-G2/PA6 (PA6) cells on dentine slices in the presence of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3]. Addition of anti-HGF neutralizing IgG to the cocultures inhibited the formation of osteoclastic cells and their dentine-resorbing activity. We detected a single 6.0-kb transcript for HGF in PA6 cells, and also recognized immunoreactive M(r) 81,000 and 88,000 forms of HGF in conditioned medium (CM) from PA6 cell cultures, the level of which reached 6 ng/ml. Both the CM and HGF stimulated the proliferation of blast cells synergistically with granulocyte-macrophage colony-stimulating factor, resulting in an increased number of osteoclast precursors that respond to 1,25(OH)2D3 that are tartrate-resistant acid phosphatase-positive multinucleate cells in stromal cell-free blast cell cultures in plastic wells. The effect of the CM was diminished by the addition of anti-HGF IgG. However, neither the CM nor HGF stimulated the formation of osteoclastic cells and pits on dentine slices in the absence of PA6 cells. These results suggest that although HGF cannot completely replace stromal cells, it is one of the paracrine mediators produced by stromal cells that act on proliferation of osteoclastic cell precursors.
机译：破骨细胞形成造血前体细胞已被证明需要基质的支持在骨组织细胞。表明肝细胞生长因子(HGF)基质细胞衍生的分子负责osteoclast-like细胞形成。在我们的实验中,我们使用一个coculture系统监测细胞的形成和激活哪些造血母细胞coculturedcalvaria-derived基质MC3T3-G2 /尼龙6(尼龙6)细胞在牙本质片的存在1, 25-dihydroxyvitamin D3 (1,25 (OH) 2 D3]。cocultures anti-HGF中和的免疫球蛋白抑制监测细胞的形成他们dentine-resorbing活动。单6.0 kb成绩单HGF在尼龙6细胞,也是公认的免疫反应性的(r) 81000和88000年形式的条件培养基中HGF从尼龙6细胞培养(CM),它的水平达到6 ng / ml。爆炸的增殖细胞表现为协同作用和集落刺激因素,导致增加的数量破骨细胞前体,回复1,25 (OH) 2 d3是tartrate-resistant酸phosphatase-positive多核的细胞用塑料基质爆炸游离细胞培养井。添加anti-HGF免疫球蛋白。和HGF刺激监测的形成细胞和牙本质片没有坑的细胞。不能完全取代基质细胞,胶质瘤的旁分泌介质产生的吗基质细胞扩散行为监测细胞的前体。

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