...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >Induction of bovine bronchial epithelial cell filopodia by tetradecanoyl phorbol acetate, calcium ionophore, and lysophosphatidic acid.
【24h】

Induction of bovine bronchial epithelial cell filopodia by tetradecanoyl phorbol acetate, calcium ionophore, and lysophosphatidic acid.

机译：牛支气管上皮细胞的诱导丝状伪足,十四酰乙酸佛波醇酯,钙离子载体和lysophosphatidic酸。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The morphological responses of primary bovine bronchial epithelial cells (BBECs) cultured in serum-free medium to protein activators have been examined. When attached to type I collagen-coated tissue culture dishes, the cells responded to tetradecanoyl phorbol acetate (TPA), calcium ionophore A23187, and lysophosphatidic acid (LPA) by extruding filopodia. In contrast, no morphological changes were elicited by exposures to either epinephrine or dibutyryl-cAMP. Formation of filopodia was accompanied by actin filament reorganization as demonstrated by staining with labeled phalloidin. Exposures to varied TPA concentrations for 2 h showed maximal stimulation of filopodial extrusions at 10 nM TPA with half-maximal stimulation at 1 nM. Time-course measurements with 10 nM TPA showed filopodia formation within 30 min of exposure, with 85% of the BBECs being filopodia positive after 5 h. Filopodia induction in 20-30% of the cells could be achieved by 1-100 microM LPA concentrations. BBECs acquired increasing resistance to TPA-induced filopodia during the initial 5 days in culture; however, responsiveness to TPA was regenerated by mild treatment with trypsin. Inclusion of fibronectin or vitronectin into the attachment matrix had no effects on the rates or extent of TPA-induced filopodia formation.
机译：主要牛的形态响应支气管上皮细胞(BBECs)培养无血清培养基蛋白质活化剂检查。组织培养,细胞反应十四酰乙酸佛波醇酯(TPA),钙离子载体A23187和lysophosphatidic酸(LPA)通过挤压丝状伪足。形态变化被曝光了肾上腺素或dibutyryl-cAMP。丝状伪足的形成是伴随着肌动蛋白丝重组了与标记phalloidin染色。不同浓度TPA 2 h显示最大在10 nM TPA刺激filopodial铝型材half-maximal刺激在1纳米。时间进程与10 nM TPA显示测量30分钟内丝状伪足形成的接触,以85%的BBECs丝状伪足阳性5 h后,丝状伪足感应的20 - 30%细胞可以通过1 - 100 microM LPA浓度。期间抵抗TPA-induced丝状伪足最初的5天的文化;TPA反应被轻微的再生用胰蛋白酶处理。或vitronectin矩阵没有附件影响利率或TPA-induced的程度丝状伪足的形成。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号