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首页> 外文期刊>Journal of Cellular Physiology >Nucleolar p120 is expressed as a delayed early response gene and is inducible by DNA-damaging agents.
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Nucleolar p120 is expressed as a delayed early response gene and is inducible by DNA-damaging agents.

机译：核仁的p120表示为延迟响应基因,诱导能损伤dna代理。

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Regulation of the expression of the growth-related nucleolar p120 protein was examined in serum-deprived and stimulated nontransformed and SV40-transformed WI-38 human fibroblasts. In quiescent cells, transcriptional activity of the p120 gene was very low or undetectable, and the steady-state levels of the p120 mRNA and the p120 protein were also negligible. The transient expression of the p120 gene in the cell cycle was detected in middle G1-phase after the expression of the early response genes and before the expression of the DNA-synthesis genes. Protein synthesis was required for the induction of p120 expression in serum-stimulated cells. The increased level of p120 mRNA in middle G1-phase was attributed to an increased transcription rate of the p120 gene, and not to a change in p120 mRNA stability. The calculated half-life of p120 mRNA was unchanged (1.8 +/- 0.2 hr) in all four cell conditions tested; i.e., in middle G1- or S-phase cells and in exponentially growing normal or transformed cells. Transcription rate of the p120 gene was correlated with the steady-state levels of either p120 protein or p120 mRNA. A sharp increase in p120 mRNA level occurred in both normal and transformed cells treated with actinomycin D used to examine p120 mRNA stability. This induction of p120 mRNA expression was seen in early G1-phase, but not in quiescent cells, or in middle to late G1-phase when cells expressed the highest level of p120 mRNA. The same expression pattern was seen by treatment with chlorambucil, another DNA-damaging agent. The conclusions of these studies are that the expression of p120 (1) is serum inducible in a fashion characteristic of the delayed early response gene products, (2) requires the presence of newly synthesized proteins, (3) is regulated transcriptionally, and (4) can be induced by DNA-damaging agents.
机译：的监管与生长有关的表达核仁的p120蛋白质检测serum-deprived和刺激是非SV40-transformed WI-38人类成纤维细胞。静止细胞的转录活动p120基因很低或检测不到,稳态的p120 mRNA和p120水平蛋白质也可以忽略不计。p120基因的表达在细胞周期在中间发现g1期后的表情早期的响应基因和前dna合成基因的表达。合成是p120所需的感应在serum-stimulated细胞表达。增加中产g1期的p120 mRNA水平是归因于增加转录率p120的基因,而不是p120的变化信使rna的稳定性。信使rna持平(1.8 + / - 0.2小时)4细胞条件测试;s阶段在指数增长的细胞和正常的或转化细胞。与稳态p120基因相关p120蛋白质或p120 mRNA水平。p120 mRNA水平发生在急剧增加正常和转化细胞治疗放线菌素D用于检查p120 mRNA稳定。被认为早在g1期,但不是在静止吗细胞,或在g1期中期到后期细胞表示p120 mRNA的最高水平。相同的表达模式被治疗苯丁酸氮芥,另一个能损伤dna的代理。这些研究的结论表达p120(1)血清诱导的时尚延迟早期的特征响应基因产物,(2)需要的存在新合成的蛋白质,(3)监管可以诱导转录,(4)dna有害。

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