Lymphocyte transendothelial migration toward smooth muscle cells in interleukin-1 beta-stimulated co-cultures is related to fibronectin interactions with alpha 4 beta 1 and alpha 5 beta 1 integrins.

Molossi S; Elices M; Arrhenius TRabinovitch M

首页> 外文期刊>Journal of Cellular Physiology >Lymphocyte transendothelial migration toward smooth muscle cells in interleukin-1 beta-stimulated co-cultures is related to fibronectin interactions with alpha 4 beta 1 and alpha 5 beta 1 integrins.

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Lymphocyte transendothelial migration toward smooth muscle cells in interleukin-1 beta-stimulated co-cultures is related to fibronectin interactions with alpha 4 beta 1 and alpha 5 beta 1 integrins.

机译：淋巴细胞transendothelial迁移到在interleukin-1平滑肌细胞beta-stimulated培养有关纤连蛋白与α4β1和交互α5β1整合蛋白。

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We previously reported infiltration of immune-inflammatory cells in coronary arteries from cardiac allografts, associated with increased endothelial and smooth muscle cell fibronectin synthesis regulated by interleukin (IL)-1 beta. We now investigate, using a porcine endothelial-smooth muscle cell co-culture system, whether IL-1 beta-stimulated fibronectin production is functionally important in lymphocyte transendothelial migration. Lymphocytes were harvested from porcine peripheral blood and, in the unactivated state or following activation with phorbol myristic acetate (PMA) and IL-2, were characterized by fluorescence-activated cell sorter (FACS) analysis and added to a confluent endothelial monolayer on the upper chamber of a transwell system. Endothelial cells, as well as smooth muscle cells (in the bottom of the chamber), were stimulated with IL-1 beta. Then transendothelial lymphocyte migration was determined in the presence of CS1 and RGD (fibronectin) peptides, blocking alpha 4 beta 1 and alpha 5 beta 1 integrin receptors on lymphocyte surfaces, respectively. A 55-70% inhibition of lymphocyte migration was observed when compared to control peptides. The combination of CS1 and RGD peptides did not significantly enhance the inhibitory effect of either peptide alone. A similar decrease in lymphocyte transendothelial migration toward smooth muscle cells was documented using a monoclonal antibody to cellular fibronectin. Furthermore, using smooth muscle cell conditioned medium, we reproduced the enhanced transendothelial lymphocyte migration as well as the inhibition with blocking peptides or fibronectin antibodies. Our data suggest that cytokine-mediated fibronectin synthesis in vascular cells recruits inflammatory cells through interactions of specific peptides with cell surface alpha 4 beta 1 and alpha 5 beta 1 integrins.

机译：我们之前报道的渗透冠状动脉免疫炎症细胞从心脏移植,联系在一起增加内皮细胞和平滑肌细胞纤连蛋白合成受白介素β1 (IL)。endothelial-smooth肌细胞培养系统,是否il - 1 beta-stimulated纤连蛋白生产功能很重要淋巴细胞transendothelial迁移。淋巴细胞是来自猪的收获外周血,或未激活的状态与佛波醇激活后十四乙酸酯(PMA)和2的特征fluorescence-activated细胞分选仪(流式细胞仪)分析和添加到汇合的内皮单层的上院transwell系统。肌肉细胞(室)的底部刺激与il - 1β。淋巴细胞迁移的决心CS1和RGD肽(纤连蛋白),阻断α4β1和α5β1整合素受体淋巴细胞表面,分别。观察移植相比,控制肽。不显著提高抑制了吗单独作用的肽。淋巴细胞减少transendothelial迁移对平滑肌细胞记录使用单克隆抗体细胞纤连蛋白。此外,通过平滑肌细胞的条件中,我们复制增强transendothelial淋巴细胞迁移以及阻断肽或抑制纤连蛋白抗体。cytokine-mediated纤连蛋白合成血管细胞新兵炎性细胞通过特定的肽的相互作用细胞表面α4β1和α5β1整合蛋白。

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来源
《Journal of Cellular Physiology》 |1995年第3期|620-633|共14页
作者
Molossi S; Elices M; Arrhenius TRabinovitch M;
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作者单位

Division of Cardiovascular Research, Hospital for Sick Children, Ontario, Canada.;

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正文语种英语
中图分类细胞生物学;
关键词
Fibronectins; arginyl-glycyl-aspartic acid; Transendothelial MigrationIntegrinsLymphocytesMuscle CellsIntegrin alpha4beta1lymphocyte migrationInterleukin-1EndotheliumIGBP1 geneSmooth muscle cellSLAMF7 geneIntegrin alpha6beta1muscles;

机译：纤连蛋白;arginyl-glycyl-aspartic酸;TransendothelialMigrationIntegrinsLymphocytesMuscle CellsIntegrinalpha4beta1lymphocyte肌肉cellSLAMF7 geneIntegrin alpha6beta1muscles;

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Lymphocyte transendothelial migration toward smooth muscle cells in interleukin-1 beta-stimulated co-cultures is related to fibronectin interactions with alpha 4 beta 1 and alpha 5 beta 1 integrins.

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