Evidence for the presence of a Hg-inhibitable water-permeability pathway and aquaporin 1 in A5 salivary epithelial cells.

Lazowski KW; Li J; Delporte CBaum BJ

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Evidence for the presence of a Hg-inhibitable water-permeability pathway and aquaporin 1 in A5 salivary epithelial cells.

机译：Hg-inhibitable的存在的证据透水性通路和水通道蛋白1在A5唾液上皮细胞。

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The molecular basis of water-permeability in salivary and other exocrine glands is not understood. We have examined two well-studied salivary epithelial cell lines for evidence of a Hg-inhibitable water-permeability pathway. A5 and HSG cells are derived from rat and human submandibular glands, respectively. Only A5 cells demonstrated such a pathway. The rate of A5 cell osmotic shrinkage was inhibited about fivefold in the presence of 300 microM HgCl2. To determine if this activity was associated with the expression of the prototypical water channel (aquaporin, AQP) AQP1, we used three separate experimental approaches; Northern analysis and reverse transcription-polymerase chain reaction (RT-PCR) analysis of isolated mRNA, and Western analysis of cell membranes. All three methods yielded positive results with A5 cells and negative results with HSG cells. The approximately 800 bp product of RT-PCR was analyzed further by sequencing and restriction enzyme digestion. The results were consistent with the previously reported coding region sequence for rat kidney AQP1. The aggregate data demonstrate that marked differences in water-permeability and water channel expression exist in these two salivary epithelial cell lines.

机译：透水性的分子基础唾液和其他外分泌腺理解。唾液上皮细胞系的证据Hg-inhibitable透水性途径。HSG细胞来自老鼠和人类分别颌下腺体。证明了这样的一个途径。渗透收缩抑制约5倍300年的存在microM HgCl2。这个活动与表达式典型的水通道(水通道蛋白,AQP1 AQP),我们使用三个独立的实验方法;聚合酶链反应(rt - pcr),分析孤立的信使rna,西方分析细胞膜。积极的结果与细胞A5和消极结果与HSG细胞。进一步分析了rt - pcr的产物测序和限制性内切酶消化。结果与之前一致对大鼠肾脏报告编码区序列AQP1。不同的透水性和水通道表达存在于这两个唾液上皮细胞系。

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来源
《Journal of Cellular Physiology》 |1995年第3期|613-619|共7页
作者
Lazowski KW; Li J; Delporte CBaum BJ;
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作者单位

Clinical Investigations and Patient Care Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892-1190, USA.;

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正文语种英语
中图分类细胞生物学;
关键词
Ion Channels; Aquaporin 1; Water-Electrolyte BalanceEpithelial Cellssynthetic dataSubmandibular GlandHSG cell lineExocrine gland;

机译：离子通道;水通道蛋白1;纠正BalanceEpithelial CellssyntheticdataSubmandibular GlandHSG细胞lineExocrine腺;

Evidence for the presence of a Hg-inhibitable water-permeability pathway and aquaporin 1 in A5 salivary epithelial cells.

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