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首页> 外文期刊>Journal of Cellular Physiology >Thrombin activates mitogen-activated protein kinase in primary astrocyte cultures.
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Thrombin activates mitogen-activated protein kinase in primary astrocyte cultures.

机译：凝血酶激活增殖作用激酶在初级星形胶质细胞的文化。

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摘要

Thrombin is known to evoke numerous inflammatory and proliferative responses in a wide variety of its target cells. Recent studies have demonstrated morphoregulatory and mitogenic effects of thrombin on astroglial cells (astrocytes). The present study deals with thrombin-induced activation of mitogen-activated protein (MAP) kinase in primary cultures of rat astrocytes. Treatment of serum-starved astrocytes with thrombin resulted in a rapid activation of tyrosine (Tyr) phosphorylation of a set of proteins including a prominent one with a molecular mass of 42 kDa (p42). The identity of p42 with MAP kinase was confirmed by MAP kinase-immunoreactivity of isolated [i.e., immunoprecipitated with anti-phosphotyrosine (PY) antibodies] p42 and by increased myelin basic protein (MBP) kinase activity present in MAP kinase immunoprecipitates of thrombin-treated cultures. Pertussis toxin (PTX) pretreatment failed to inhibit thrombin stimulation of p42 phosphorylation, indicating the lack of involvement of PTX sensitive G proteins in the mechanism of activation of MAP kinase by thrombin. Chronic exposure of cultures to phorbol 12-myristate 13-acetate to down-regulate PKC resulted in an attenuation of thrombin-induced p42 Tyr phosphorylation, although H-7, a known PKC inhibitor, failed to block thrombin effect. However, staurosporine, a nonspecific protein kinase inhibitor, prevented the activation of p42 phosphorylation. It is concluded that thrombin induces MAP kinase activation in astrocytes by a mechanism involving a staurosporine-sensitive pathway.
机译：凝血酶是唤起许多炎症并在各种增生性反应它的靶细胞。morphoregulatory和促有丝分裂的凝血酶对astroglial细胞的影响(星形胶质细胞)。thrombin-induced激活增殖作用蛋白质(MAP)激酶在老鼠的主要文化星形胶质细胞。与凝血酶导致迅速激活酪氨酸磷酸化(酪氨酸)一套蛋白质包括著名的一个42 kDa的分子质量(第42页)。第42页与MAP激酶证实了地图kinase-immunoreactivity孤立的(例如,免疫沉淀反应与anti-phosphotyrosine (PY)抗体)第42页和髓鞘基本增加蛋白(MBP)激酶活性出现在地图上激酶免疫沉淀反应的thrombin-treated文化。未能抑制凝血酶刺激第42页磷酸化,说明缺乏PTX敏感的G蛋白的参与MAP激酶的活化机理凝血酶。12-myristate 13-acetate下调PKC导致thrombin-induced的衰减第42页酪氨酸磷酸化,虽然第7,一个已知的PKC抑制剂,未能阻止凝血酶的影响。然而,staurosporine,特异性的蛋白质激酶抑制剂,防止激活页磷酸化。诱发MAP激酶在星形胶质细胞的激活机制涉及staurosporine-sensitive途径。

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