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首页> 外文期刊>Journal of Cellular Physiology >Cell cycle variation of Hsp70 levels in HeLa cells at 37 degrees C and after a heat shock.
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Cell cycle variation of Hsp70 levels in HeLa cells at 37 degrees C and after a heat shock.

机译：细胞周期变化的海拉细胞Hsp70水平在37摄氏度和热休克。

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摘要

The expression of the 72 kD inducible heat shock protein (hsp72) has been reported to be cell cycle associated in unheated, synchronized HeLa cells. In this study, flow cytometry was used to investigate hsp72 levels through the cell cycle in HeLa cells by dual labeling with propidium iodide and antibodies against hsp72. The entire cell cycle distribution of hsp72 could be measured in a single sample of asynchronously growing cells. For unheated cells, the level of hsp72 increased about 30% from G1 to S phase, with about a 65% increase in G2/M, probably due to cell size differences. Neither mitotic selection nor serum stimulation induced a higher level of hsp72 than in the control cells. Western blot analysis of hsp72 from Hoechst-stained cells sorted from G1, mid-S, or G2/M showed that G1 cells had the lowest level of hsp72, with about a 30% increase in S phase and a 60% increase in G2/M, in good agreement with the flow cytometry results. These data conflict with previous reports by other laboratories which showed a 3-fold higher level of hsp72 in S phase than in G1 or G2. In contrast, heat shock (both acute and chronic) led to a non-uniform increase in hsp72 through the cell cycle. Most cells in mid S phase had high levels of hsp72, and a larger range in the levels of hsp72 were found in G1 and late S/G2/M phase cells.
机译：72 kD的表达诱导热休克蛋白质(hsp72)细胞周期相关的在没有暖气的,海拉同步细胞。通过细胞周期研究hsp72水平在海拉细胞与propidium双重标签碘和hsp72抗体。细胞周期分布hsp72以一个异步的样本细胞生长。从G1 S期hsp72增加约30%,有G2 / M增加65%,可能由于细胞大小的差异。选择和血清刺激诱导更高hsp72水平比在控制细胞。从Hoechst-stained污点分析hsp72细胞排序从G1、mid-S或G2 / M表明G1细胞hsp72水平最低,约为S期增长30%,增加了60%G2 / M,在良好的协议与流式细胞术结果。其他实验室的报告显示三倍更高层次的hsp72在S期G1和G2。慢性hsp72增加)导致不均匀通过细胞周期。有高水平的hsp72,和一个更大的范围hsp72的水平被发现在G1和迟到S / G2 / M期细胞。

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