Regulation of DNA methyltransferase during differentiation of F9 mouse embryonal carcinoma cells.

Teubner B; Schulz WA

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Regulation of DNA methyltransferase during differentiation of F9 mouse embryonal carcinoma cells.

机译：监管期间DNA甲基转移酶F9老鼠胚胎性癌的分化细胞。

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DNA becomes demethylated when F9 mouse embryonal carcinoma cells differentiate into parietal endoderm. DNA methyltransferase (DNA-MTase) activity decreased by 50% during 1 week of differentiation. The level of DNA-MTase mRNA was also diminished accordingly, but the transcription rate of the DNA-MTase gene measured by run-on transcription was essentially unchanged, indicating regulation of DNA-MTase expression at a posttranscriptional step. The decline of DNA-MTase mRNA paralleled that of histone H3 mRNA in accord with the notion that DNA-MTase is preferentially expressed in the S phase of the cell cycle. Since DNA-MTase expression decreases in parallel with DNA synthesis, DNA demethylation during differentiation of F9 cells appears not to be due to limited expression of DNA-MTase. However, the plasmid pAFP7000CAT, alpha-fetoprotein (AFP), which is strongly de novo methylated when transfected into F9 stem cells became only weakly methylated after transfection into the F9 parietal endoderm derivative P1,indicating that the activity of DNA-MTase within parietal endoderm cells is more strongly diminished than is apparent from measurements of mRNA amounts and of overall DNA-MTase activity in vitro. The discrepancy between DNA-MTase expression and its actual activity within the cell indicates the existence of a novel mechanism controlling the activity of DNA-MTase.

机译：DNA时F9成了脱甲基鼠胚癌细胞分化成壁endoderm .活动在1周下降了50%分化。也相应减少,但DNA-MTase基因的转录率测量由流水转录本质上是不变,表明DNA-MTase监管表达在转录后的一步。DNA-MTase mRNA的平行的衰落组蛋白H3 mRNA在符合的观念DNA-MTase优先表达的年代阶段的细胞周期。表达降低与DNADNA合成、脱甲基作用期间F9细胞的分化,似乎并不是由于DNA-MTase有限的表达。质粒pAFP7000CAT,甲胎蛋白(AFP),强烈从头甲基化时是哪一个转染F9干细胞成为弱甲基化后转染F9顶叶内胚层导数P1,表明内DNA-MTase顶叶的活动内胚层细胞更强烈减弱明显是测量信使rna数量和人吗整体DNA-MTase活动体外。DNA-MTase表达式及其之间的差异细胞内的实际活动表示存在的一种新颖的控制机制DNA-MTase的活动。

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来源
《Journal of Cellular Physiology》 |1995年第2期|284-290|共7页
作者
Teubner B; Schulz WA;
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作者单位

Institut fur Physiologische Chemie I, Heinrich-Heine Universitat, Dusseldorf, Germany.;

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原文格式 PDF
正文语种英语
中图分类细胞生物学;
关键词
Methyltransferases; DNA Modification Methylases; S PhaseEmbryonal Carcinoma Stem CellsmessengersF9 Teratocarcinoma Stem CellsCarcinomaEndodermDNARegulation (biology)RNAF9 cell lineIndividualized InstructionMessenger RNA;

机译：甲基转移酶、DNA甲基化酶修改PhaseEmbryonal癌干细胞CellsmessengersF9畸胎癌干细胞CellsCarcinomaEndodermDNARegulation(生物学)lineIndividualized RNAF9细胞InstructionMessenger RNA;

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Regulation of DNA methyltransferase during differentiation of F9 mouse embryonal carcinoma cells.

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