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首页> 外文期刊>Journal of Cellular Physiology >Induction of P-glycoprotein mRNA by protein synthesis inhibition is not controlled by a transcriptional repressor protein in rat and human liver cells.
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Induction of P-glycoprotein mRNA by protein synthesis inhibition is not controlled by a transcriptional repressor protein in rat and human liver cells.

机译：感应22 mRNA的蛋白质合成抑制并不是由一个控制在老鼠和转录阻遏蛋白质人类肝细胞。

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Recent studies have suggested that a labile transcriptional repressor protein is important in the regulation of pgp mRNA expression. However, cycloheximide (CHX) the protein synthesis inhibitor used, can increase mRNAs by either stabilizing the mRNA transcript or directly activating gene transcription. To determine whether CHX posttranscriptionally increased pgp mRNA, we compared the effect of CHX, which inhibits protein synthesis by stabilizing polysomes, with puromycin (PURO), which inhibits protein synthesis by polysome destabilization. In rat hepatocytes, CHX induced pgp2 mRNA, and the increase was proportional to the degree of protein synthesis inhibition. In contrast, despite almost complete inhibition of protein synthesis, PURO did not induce pgp2 mRNA. Further studies demonstrated that PURO pretreatment could block pgp2 mRNA induction by CHX. Likewise, in cultures of primary human hepatocytes CHX, but not PURO, induced MDR1 mRNA. A polymerase chain reaction assay was developed to assess whetherCHX treatment altered the length of the 3'-untranslated region (UTR) of pgp2. CHX treatment time dependently increased the length of the pgp2 3'-UTR. To determine whether CHX acts as a transcriptional agonist, we performed nuclear run-off analysis and found no increase in pgp2 gene transcription compared to untreated control. Further, transcription studies were performed by transiently transfecting HepG2 cells with plasmids containing 5' segments of human MDR1 fused with the reporter chloramphenicol acetyltransferase (CAT). These plasmids were not transcriptionally activated by CHX. In summary, our results cast doubt on the existence of a labile transcriptional repressor protein for pgp. Furthermore, these are the first studies to demonstrate that polysomal destabilization by PURO can block CHX induction of pgp.
机译：最近的研究表明,不稳定转录阻遏蛋白质是重要的pgp mRNA表达的调节。环己酰亚胺(CHX)蛋白质合成抑制剂使用,可以增加mrna稳定mRNA转录或直接激活基因转录。是否CHX转录后的pgp增加信使rna,我们CHX的影响相比,哪个抑制蛋白质合成的稳定多核糖体,嘌呤霉素(嘌呤霉素),抑制多核糖体蛋白质合成的不稳定。大鼠肝细胞,CHX诱导pgp2 mRNA,增加的程度成正比抑制蛋白质的合成。尽管几乎完全抑制的蛋白质合成、雪茄烟没有诱导pgp2信使rna。研究表明,嘌呤霉素预处理块pgp2 mRNA CHX感应。文化的主要人类肝细胞CHX,但是不是雪茄烟,诱导凋亡信使rna。反应分析是评估whetherCHX开发的治疗改变的长度(UTR) pgp2 3 '端非翻译区。治疗时间依赖性的长度pgp2 3’utr。作为一个转录兴奋剂,我们执行核径流分析,发现没有增加pgp2比未经处理的基因转录控制。由瞬变使转染HepG2细胞与质粒含有5 '的人凋亡融合记者氯霉素乙酰转移酶(CAT)。由CHX转录激活。我们的结果质疑的存在不稳定的转录阻遏蛋白pgp。此外,这是第一个研究证明polysomal扰动嘌呤霉素可以阻止CHX pgp的感应。

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