...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >Regulation of apoptosis of interleukin 2-dependent mouse T-cell line by protein tyrosine phosphorylation and polyamines.
【24h】

Regulation of apoptosis of interleukin 2-dependent mouse T-cell line by protein tyrosine phosphorylation and polyamines.

机译：调控细胞凋亡的白介素2-dependent小鼠t细胞逐蛋白质酪氨酸磷酸化和多胺。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We examined the effect of inhibitors of tyrosine kinase and tyrosine phosphatase on DNA fragmentation, protein tyrosine phosphorylation, and polyamine metabolism in the murine T-cell line CTLL-2. When cells were exposed to herbimycin A, a specific inhibitor of tyrosine kinase (Uehara et al., 1989, Biochem. Biophys. Res. Commun., 163:803-809), in the presence of interleukin 2 (IL-2), DNA was degraded into oligonucleosomal fragments in a dose-dependent fashion. Genistein, another inhibitor of tyrosine kinase (Akiyama et al., 1987, J. Biol. Chem., 262:5592-5596), had similar effects. Exposure of CTLL-2 cells to vanadate, a tyrosine phosphatase inhibitor, blocked with the DNA fragmentation induced by herbimycin A. Tyrosine phosphorylation of 55 Kd protein was inhibited by herbimycin A, and the inhibition was reduced by vanadate. Ornithine decarboxylase (ODC) activity decreased rapidly after herbimycin A was added to CTLL-2 cell cultures, while vanadate increased ODC activity. The exogenous addition of putrescine or spermine, but not that of spermidine, attenuated herbimycin A-induced DNA fragmentation. These findings suggest that phosphorylation of tyrosine residues of 55 Kd protein prevents DNA fragmentation and that polyamines are involved in regulation of apoptosis.
机译：我们检查了酪氨酸的抑制剂的影响激酶,酪氨酸磷酸酶的DNA碎片,蛋白质酪氨酸磷酸化,和聚胺小鼠t细胞的新陈代谢行CTLL-2。herbimycin,特定抑制剂酪氨酸kinase (Uehara科幻片阿尔。、1989、Biochem。Commun >,白介素2 (2),DNA降解oligonucleosomal碎片存在剂量依赖的相关性时尚。kinase (Akiyama科幻片阿尔。1987,,j。Biol。262:5592 - 5596),也有类似的效果。钒酸CTLL-2细胞酪氨酸磷酸酶抑制剂,阻止DNA碎片引起herbimycin酪氨酸磷酸化55 Kd蛋白是由herbimycin抑制,和钒酸抑制降低了。鸟氨酸脱羧酶(ODC)活性降低之后迅速herbimycin CTLL-2添加ODC钒酸细胞培养,而增加活动。精胺,但不是亚精胺,减毒herbimycin A-induced DNA碎片。研究结果表明,酪氨酸的磷酸化残留的55 Kd蛋白阻止DNA碎片,并参与多胺调节细胞凋亡。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号