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首页> 外文期刊>Journal of Cellular Physiology >Expression and regulation of superoxide dismutase activity in human skin fibroblasts from donors of different ages.
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Expression and regulation of superoxide dismutase activity in human skin fibroblasts from donors of different ages.

机译：超氧化物歧化酶的表达和调控从捐赠者的人类皮肤成纤维细胞的活动不同的年龄。

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摘要

We have determined the activities, protein, and mRNA abundances as well as the level of transcriptional activation of two intracellular forms of the free radical metabolizing enzyme superoxide dismutase in 29 human skin fibroblast lines established from donors of different ages. SOD-1 (a copper and zinc containing form of SOD) and SOD-2 (a manganese containing form of the enzyme) activities were both observed to be significantly lower in cell lines derived from fetal skin than in lines established from postnatal skin (ages 17-94 years). The percent of total activity contributed by SOD-1 decreased in an age-associated manner from approximately 50% in the fetal lines to less than 20% in lines established from old tissue donors. All of the cell lines were screened to exclude the possibility that they contained a polymorphism known to influence SOD-2 activity. Northern blot analysis revealed three SOD-1 mRNA transcripts that were 0.5, 0.7, and 1.9 kb in length. Although SOD-1 protein abundance was lower infetal lines than in lines derived from postnatal donors, SOD-1 mRNA abundance did not differ between fetal cells and cell lines derived from young donors. SOD-2 protein abundance and mRNA abundance were both significantly lower in fetal lines than in postnatal lines. No postnatal age-dependent differences were observed in any of the SOD-2 parameters examined. Nuclear run-on analysis revealed that fetal cell lines exhibited a lower level of transcriptional initiation for SOD-1 than postnatal lines. The transcription of SOD-2 was readily detected in postnatal lines, but undetectable in fetal lines. These results are consistent with multiple levels of control for SOD-1 expression and with a strong transcriptional influence on SOD-2 expression.
机译：我们已经确定的活动,蛋白质,信使rna含量,以及水平转录激活两种细胞内形式的自由基代谢酶超氧化物歧化酶在29个人类皮肤成纤维细胞行建立了不同年龄的捐赠者。SOD-1(一种含铜和锌的SOD)和SOD-2(包含形式的锰酶)活动都观察到在细胞系来源于显著降低胎儿的皮肤比线建立产后皮肤(17 - 94岁)。总活动由SOD-1减少大约有50%的与年龄有关的方式在不到20%的胎儿行线建立了从旧组织捐助者。细胞系筛选排除他们包含一个多态的可能性影响SOD-2活动。分析显示三个SOD-1 mRNA转录0.5、0.7和1.9 kb。尽管SOD-1蛋白质丰度较低来自infetal线比行产后捐助者、SOD-1 mRNA丰度没有胎儿细胞之间和不同细胞系从年轻的捐助者。信使rna丰度都显著降低比产后胎儿行线。年龄相关性差异的观察SOD-2参数检查。分析显示,胎儿细胞系表现出的转录起始级别较低比产后SOD-1线。SOD-2在产后容易检测到线,但是在胎儿行察觉。符合多个级别的控制SOD-1表达式和强大转录的影响SOD-2表达式。

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