...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >Adenine nucleotide metabolism by chondrocytes in vitro: role of ATP in chondrocyte maturation and matrix mineralization.
【24h】

Adenine nucleotide metabolism by chondrocytes in vitro: role of ATP in chondrocyte maturation and matrix mineralization.

机译：腺嘌呤核苷酸代谢的软骨细胞ATP的软骨细胞的成熟和体外:角色矩阵的矿化。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The objective of the investigation was to explore the notion that chondrocytes in the growth plate secrete nucleotides and that these compounds are used to regulate cell maturation and matrix mineralization. Chondrocytes were isolated from the cephalic region of chick embryo sterna and maintained in culture until confluent. To promote expression of the mature phenotype, cultures were then treated with retinoic acid. During the culture period, medium was removed and analyzed for nucleotides using a modified reverse-phase high-performance liquid chromatography (HPLC) procedure. We found that culture medium, conditioned by the chondrocytes, contained significant quantities of nucleotides. Moreover, the nucleotide concentrations were similar in magnitude to levels reported for media conditioned by other cell types. In terms of species, adenosine diphosphate (ADP) was the major nucleotide present in the conditioned medium; adenosine monophosphate (AMP) was present, but at a lower concentration than ADP. To examine the possibility that adenosine triphosphate (ATP) was released by the cultured chondrocytes, but was rapidly degraded into ADP and AMP, we examined the kinetics of ATP breakdown by chondrocytes. We found that chondrocytes degraded over 70% of exogenous ATP within 15 minutes. Similar experiments performed with ADP and AMP indicated that these nucleotides were also degraded by the cells, but at a slower rate than ATP. To determine whether the extracellular nucleotides modulate cartilage development, we examined the effect of exogenous ATP on four major determinants of chondrocyte function: alkaline phosphatase activity, cell proliferation rate, anaerobic metabolism, and mineral deposition. We found that ATP caused only minimum alterations in cell number and alkaline phosphatase activity; however, it increased the lactate content of the medium probably by stimulating anaerobic glycolysis. We noted that ATP had a significant effect on the amount and type of mineral deposited into chondrocyte cultures. Compared with untreated controls, ATP stimulated formation of a small amount of poorly crystallized calcium phosphate. The results of the study show for the first time that chondrocytes release nucleotides into the extracellular milieu. Although they are rapidly degraded, they serve to regulate both mineral formation and energy metabolism.
机译：调查的目的是为了探索在生长板软骨细胞的概念分泌核苷酸,这些化合物用于调节细胞成熟和矩阵矿化。小鸡胚胎头地区的胸骨维护文化直到汇合的。成熟的表型的表达、文化然后用维甲酸治疗。文化时期,介质被和分析核苷酸使用修改后的反相高效液相色谱法(HPLC)过程。软骨细胞的条件,控制大量的核苷酸。核苷酸浓度相似级水平对媒体报道受制于其他细胞类型。物种,二磷酸腺苷(ADP)主要的条件中出现的核苷酸媒介;存在,但比ADP浓度较低。检查腺苷的可能性三磷酸腺苷(ATP)发布的讲究的软骨细胞,而是迅速退化成ADPAMP,我们检查了ATP的动力学软骨细胞分解。软骨细胞退化外源性ATP的70%以上在15分钟。ADP、AMP表示,这些核苷酸也是由细胞退化,但以较慢的比ATP。细胞外核苷酸调节软骨发展,我们检查了外生的效果ATP四软骨细胞的主要决定因素功能:碱性磷酸酶活性,细胞增殖率、厌氧代谢和矿物沉积。最小改变细胞数量和碱性磷酸酶活动;乳酸中可能的内容刺激无氧糖酵解。ATP和数量有显著影响类型的矿物沉积成软骨细胞文化。刺激形成少量的差磷酸钙结晶。这项研究首次展示软骨细胞释放核苷酸进入细胞外环境。退化,他们用来调节两种矿物形成和能量代谢。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号