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首页> 外文期刊>Journal of Cellular Physiology >Retinoic acid nuclear receptor beta inhibits breast carcinoma anchorage independent growth.
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Retinoic acid nuclear receptor beta inhibits breast carcinoma anchorage independent growth.

机译：视黄酸核受体β抑制乳腺癌锚地独立成长。

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摘要

Retinoids modulate cellular proliferation and mediate gene function through a series of nuclear receptors. The retinoic acid nuclear receptor beta (RAR beta) plays an important role in the differentiation of a number of cell types. We now demonstrate that RAR beta expression is confined to normal mammary tissue and is not expressed in either immortalized normal or malignant cell lines. Treatment of RAR beta-transfected MDA-MB-231 cells with 1 microM all-trans-retinoic acid (RA) significantly inhibited monolayer growth of the cells which express recombinant RAR beta. RAR beta-expressing MDA-MB-231 cells formed significantly smaller and fewer colonies in soft agar than the mock-transfected cells. Addition of 1 microM RA stimulated colony size and number in the RAR beta-transfected MDA-MB-231 cells. In contrast to the RAR beta-expressing cells, colony formation by the RAR alpha-expressing cells was similar to the mock-transfected controls and the addition of 1 microM RA to the RAR alpha-transfected cells inhibited colony formation. While demonstrating decreased colony formation in agar, RAR beta-expressing MDA-MB-231 cells failed to exhibit decreased growth in SCID mice. Our results show that RAR beta functions as a negative regulator of growth in breast epithelial cells. In addition, the growth of these cells is differentially regulated by RAR alpha and RAR beta which is most likely the result of the modulation of different genes.
机译：类维生素a调节细胞增殖和调节基因通过一系列的核函数受体。ββ(RAR)中发挥着重要作用分化的细胞类型。证明RARβ表达局限正常乳腺组织不表达不灭的正常或恶性肿瘤细胞行。mda - mb - 231细胞1 microM all-trans-retinoic酸(RA)显著抑制单层增长的细胞表达重组RARβ。在软明显较小的和更少的殖民地琼脂比mock-transfected细胞。1 microM RA刺激菌落的大小和数量RAR beta-transfected mda - mb - 231细胞。RAR beta-expressing细胞相比,殖民地RAR alpha-expressing细胞形成类似于mock-transfected控制和1 microM RA的RARalpha-transfected细胞抑制殖民地形成。形成在琼脂,RAR beta-expressing mda - mb - 231细胞没有表现出减少SCID的增长老鼠。消极的监管机构的乳腺癌的增长上皮细胞。这些细胞被RAR差别监管α和βRAR是最有可能的不同基因的调制的结果。

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