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首页> 外文期刊>EMBO Journal >Helicase-dependent isothermal DNA amplification
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Helicase-dependent isothermal DNA amplification

机译：Helicase-dependent等温DNA扩增

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摘要

Polymerase chain reaction is the most widely used method for in vitro DNA amplification. However, it requires thermocycling to separate two DNA strands. In vivo, DNA is replicated by DNA polymerases with various accessory proteins, including a DNA helicase that acts to separate duplex DNA. We have devised a new in vitro isothermal DNA amplification method by mimicking this in vivo mechanism. Helicase-dependent amplification (HDA) utilizes a DNA helicase to generate single-stranded templates for primer hybridization and subsequent primer extension by a DNA polymerase. HDA does not require thermocycling. In addition, it offers several advantages over other isothermal DNA amplification methods by having a simple reaction scheme and being a true isothermal reaction that can be performed at one temperature for the entire process. These properties offer a great potential for the development of simple portable DNA diagnostic devices to be used in the field and at the point-of-care.
机译：聚合酶链反应是使用最广泛的方法体外DNA扩增。它需要热循环分离两个DNA链。聚合酶与各种辅助蛋白质,包括DNA解旋酶分离双螺旋DNA。通过模拟等温DNA扩增方法这种体内机制。利用DNA解旋酶放大()注重科技进步为引物生成单链模板杂交和随后的引物延伸一个DNA聚合酶。热循环。优于其他等温DNA通过一个简单的反应放大方法方案和成为一个真正的等温反应在一个温度可以执行吗整个过程。简单便携的发展潜力DNA诊断设备中使用在现阶段。

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