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首页> 外文期刊>Ophthalmic surgery, lasers & imaging: the official journal of the International Society for Imaging in the Eye >In vivo laser confocal microscopic analysis of murine cornea and lens microstructures.
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In vivo laser confocal microscopic analysis of murine cornea and lens microstructures.

机译：体内激光共焦显微镜分析小鼠角膜和晶状体微观结构。

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摘要

BACKGROUND AND OBJECTIVE: The purpose of the current study is to investigate in vivo microstructures of anterior segments of normal murine eyes by new-generation in vivo laser confocal microscopy. MATERIALS AND METHODS: Twenty-six corneas and lenses from 13 mice were analyzed by in vivo laser confocal microscopy. RESULTS: Murine corneal superficial cells formed a polygonal cell pattern, with a mean cell density of 577 +/- 115 cells/mm2 (mean +/- standard deviation). Corneal basal epithelial cells had dark cytoplasm and were closely organized (9,312 +/- 1,777 cells/mm2). Sub-basal nerve fiber bundles were arranged in a whorl pattern, with both clockwise and counter-clockwise patterns. In the stroma, keratocytes were observed as numerous reflective stellate structures. The endothelial cells were organized in a honeycomb pattern (2,463 +/- 292 cells/mm2). Deeper inside the eye, murine lens epithelial cells were organized in a regular pattern (4,168 +/- 636 cells/mm2) and numerous lens fibers were observed. CONCLUSION: In vivo laser confocal microscopy can provide high-resolution images of all corneal layers and lens structures of mice without sacrificing animals or tissue preparation.
机译：背景和目的:的目的目前的研究调查体内前部分的微观结构正常小鼠体内新一代激光眼睛共焦显微镜。26从13个老鼠眼角膜和镜头分析了体内激光共焦显微镜。结果:小鼠角膜表面的细胞形成一个多边形细胞模式,平均细胞577 + / - 115细胞密度/平方毫米(意思是+ / -标准偏差)。细胞有黑暗的细胞质和密切组织(9312 + / - 1777细胞/平方毫米)。神经纤维束排列在一个螺纹模式,顺时针方向和逆时针方向的模式。观察,可见大量的反光星状结构。组织在一个蜂窝模式(2463 + / - 292细胞/平方毫米)。上皮细胞在定期组织模式(4168 + / - 636细胞/平方毫米)和无数晶状体纤维。激光共焦显微镜可以提供角膜层和高分辨率图像镜头结构的老鼠不牺牲动物或组织准备。

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