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首页> 外文期刊>Acta crystallographica. Section D, Biological crystallography. >Structure of Csd3 from Helicobacter pylori, a cell shape-determining metallopeptidase
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Structure of Csd3 from Helicobacter pylori, a cell shape-determining metallopeptidase

机译：从幽门螺杆菌Csd3结构,细胞shape-determining metallopeptidase

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摘要

Helicobacter pylori is associated with various gastrointestinal diseases such as gastritis, ulcers and gastric cancer. Its colonization of the human gastric mucosa requires high motility, which depends on its helical cell shape. Seven cell shape-determining genes (csd1, csd2, csd3/hdpA, ccmA, csd4, csd5 and csd6) have been identified in H. pylori. Their proteins play key roles in determining the cell shape through modifications of the cell-wall peptidoglycan by the alteration of cross-linking or by the trimming of peptidoglycan muropeptides. Among them, Csd3 (also known as HdpA) is a bifunctional enzyme. Its D , D -endopeptidase activity cleaves the D -Ala4-mDAP3 peptide bond between cross-linked muramyl tetrapeptides and pentapeptides. It is also a D , D -carboxypeptidase that cleaves off the terminal D -Ala5 from the muramyl pentapeptide. Here, the crystal structure of this protein has been determined, revealing the organization of its three domains in a latent and inactive state. The N-terminal domain 1 and the core of domain 2 share the same fold despite a very low level of sequence identity, and their surface-charge distributions are different. The C-terminal LytM domain contains the catalytic site with a Zn2+ ion, like the similar domains of other M23 metallopeptidases. Domain 1 occludes the active site of the LytM domain. The core of domain 2 is held against the LytM domain by the C-terminal tail region that protrudes from the LytM domain.
机译：幽门螺杆菌与各种相关胃肠道疾病,如胃炎、溃疡和胃癌。人类的胃粘膜需要高能动性,这取决于它的螺旋形细胞形状。细胞shape-determining基因(csd1 csd2,csd3 / hdpA ccmA、csd4 csd5和csd6)发现幽门螺旋杆菌。角色在决定细胞的形状修改的细胞壁肽聚糖交联或变更修剪的肽聚糖muropeptides。他们,Csd3(也称为HdpA)是一个双功能酶。D -Ala4-mDAP3肽键之间交联胞壁四肽和消除皱纹。羧肽酶,劈开了终端D-Ala5胞壁五肽。这种蛋白质的晶体结构确定,揭示组织的三个领域潜在的和不活跃的状态。1 n端结构域和域的核心2共享相同的褶皱,尽管一个非常低的水平序列的身份,和他们的表面电荷分布是不同的。域包含Zn2 +催化部位离子,像其他类似的领域M23一样metallopeptidases。LytM域。针对c端LytM域的举行尾部区域,突出于LytM域。

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