S-SAD phasing of monoclinic histidine kinase from Brucella abortus combining data from multiple crystals and orientations: an example of data-collection strategy and a posteriori analysis of different data combinations

Klinke Sebastian; Foos Nicolas; Rinaldi Jimena J.Paris GastonGoldbaum Fernando A.Legrand PierreGuimaraes Beatriz G.Thompson Andrew

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >S-SAD phasing of monoclinic histidine kinase from Brucella abortus combining data from multiple crystals and orientations: an example of data-collection strategy and a posteriori analysis of different data combinations

【24h】

S-SAD phasing of monoclinic histidine kinase from Brucella abortus combining data from multiple crystals and orientations: an example of data-collection strategy and a posteriori analysis of different data combinations

机译：遗憾定相的单斜组氨酸激酶流产布鲁氏菌结合来自多个数据晶体取向:一个例子数据收集策略和后验分析不同的数据组合

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

The histidine kinase (HK) domain belonging to the light-oxygen-voltage histidine kinase (LOV-HK) from Brucella abortus is a member of the HWE family, for which no structural information is available, and has low sequence identity (20%) to the closest HK present in the PDB. The 'off-edge' S-SAD method in macromolecular X-ray crystallography was used to solve the structure of the HK domain from LOV-HK at low resolution from crystals in a low-symmetry space group (P2(1)) and with four copies in the asymmetric unit (similar to 108 kDa). Data were collected both from multiple crystals (diffraction limit varying from 2.90 to 3.25 angstrom) and from multiple orientations of the same crystal, using the kappa-geometry goniostat on SOLEIL beamline PROXIMA 1, to obtain 'true redundancy'. Data from three different crystals were combined for structure determination. An optimized HK construct bearing a shorter cloning artifact yielded crystals that diffracted X-rays to 2.51 angstrom resolution and that were used for final refinement of the model. Moreover, a thorough a posteriori analysis using several different combinations of data sets allowed us to investigate the impact of the data-collection strategy on the success of the structure determination.

机译：组氨酸激酶(香港)域属于light-oxygen-voltage组氨酸激酶(LOV-HK)流产布鲁氏菌属于HWE家庭,没有结构信息可用,序列的身份较低(20%)最接近香港出现在PDB。遗憾在大分子x射线方法晶体学是用来解决结构在低分辨率的香港域LOV-HK从晶体low-symmetry空间群(P2(1))和不对称的四份单位(类似于108 kDa)。从多个晶体(衍射极限从2.90到3.25埃)和不同多个方向相同的水晶,使用的kappa-geometry goniostat SOLEIL beamline比邻星,获得真正的冗余。三个不同的晶体结合了结构的决心。建立轴承工件较短的克隆产生x射线晶体衍射,至2.51埃分辨率和用于最终模型的改进。使用几种不同的后验分析数据集允许我们的组合调查收集的影响战略成功的结构的决心。

著录项

来源
《Acta crystallographica.Section D. Biological crystallography》 |2015年第7期|1433-1443|共11页
作者
Klinke Sebastian; Foos Nicolas; Rinaldi Jimena J.Paris GastonGoldbaum Fernando A.Legrand PierreGuimaraes Beatriz G.Thompson Andrew;
展开▼
作者单位

IIBBA CONICET, Fdn Inst Leloir, Buenos Aires, DF, Argentina;

Synchrotron SOLEIL, Expt Div, F-91192 Gif Sur Yvette, France;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类
关键词
crystals; Binary systems; Histidine Protein KinaseBrucella abortusDataset;

机译：晶体;二进制系统;组氨酸蛋白;

S-SAD phasing of monoclinic histidine kinase from Brucella abortus combining data from multiple crystals and orientations: an example of data-collection strategy and a posteriori analysis of different data combinations

摘要

著录项

相关主题

期刊订阅