...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Multi-crystal native SAD analysis at 6 keV
【24h】

Multi-crystal native SAD analysis at 6 keV

机译：多晶本机6 keV悲伤的分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Anomalous diffraction signals from typical native macromolecules are very weak, frustrating their use in de novo structure determination. Here, native SAD procedures are described to enhance signal to noise in anomalous diffraction by using multiple crystals in combination with synchrotron X-rays at 6 keV. Increased anomalous signals were obtained at 6 keV compared with 7 keV X-ray energy, which was used for previous native SAD analyses. A feasibility test of multicrystal-based native SAD phasing was performed at 3.2 angstrom resolution for a known tyrosine protein kinase domain, and real-life applications were made to two novel membrane proteins at about 3.0 angstrom resolution. The three applications collectively serve to validate the robust feasibility of native SAD phasing at lower energy.
机译：从典型的原生异常衍射信号大分子非常弱,令人沮丧的自己使用新创结构的决心。本机悲伤过程描述增强在反常衍射信号噪声多个晶体结合同步加速器x射线keV 6点。获得6点keV相比7 keV x射线能源,用于前本地悲伤分析。multicrystal-based原生悲伤定相为一个已知的表现为3.2埃分辨率酪氨酸蛋白激酶域和真实应用程序是由两个新颖的膜蛋白质约为3.0埃分辨率。三个应用程序共同验证服务本机悲伤逐步的健壮的可行性较低的能量。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号