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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Pushing the limits of sulfur SAD phasing: de novo structure solution of the N-terminal domain of the ectodomain of HCV E1
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Pushing the limits of sulfur SAD phasing: de novo structure solution of the N-terminal domain of the ectodomain of HCV E1

机译：推动硫悲伤的限制逐步:新创结构的n端结构域的解决方案丙肝病毒E1的ectodomain

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摘要

Single-wavelength anomalous dispersion of S atoms (S-SAD) is an elegant phasing method to determine crystal structures that does not require heavy-atom incorporation or selenomethionine derivatization. Nevertheless, this technique has been limited by the paucity of the signal at the usual X-ray wavelengths, requiring very accurate measurement of the anomalous differences. Here, the data collection and structure solution of the N-terminal domain of the ectodomain of HCV E1 from crystals that diffracted very weakly is reported. By combining the data from 32 crystals, it was possible to solve the sulfur substructure and calculate initial maps at 7 angstrom resolution, and after density modication and phase extension using a higher resolution native data set to 3.5 angstrom resolution model building was achievable.
机译：单波长反常色散原子的年代(遗憾)是一个优雅的分阶段方法来确定晶体结构,不需要重原子合并或硒代蛋氨酸衍生化。被缺乏有限的信号通常的x射线波段,要求非常准确测量异常的差异。数据收集和结构的解决方案n端结构域的ectodomain丙肝病毒E1从晶体衍射非常弱报道。有可能解决硫子结构7点和计算初始地图埃密度modication和分辨率,和之后使用高分辨率原生扩展阶段数据集到3.5埃分辨率模型建筑是可以实现的。

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