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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Latent and active abPPO4 mushroom tyrosinase cocrystallized with hexatungstotellurate(VI) in a single crystal
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Latent and active abPPO4 mushroom tyrosinase cocrystallized with hexatungstotellurate(VI) in a single crystal

机译：潜伏性和活动性abPPO4蘑菇酪氨酸酶共晶与hexatungstotellurate (VI)单晶

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摘要

Tyrosinases, bifunctional metalloenzymes, catalyze the oxidation of monophenols and o-diphenols to o-quinones, the precursor compounds of the brown-coloured pigment melanin. In eukaryotic organisms, tyrosinases are expressed as latent zymogens that have to be proteolytically cleaved in order to form highly active enzymes. This activation mechanism, known as the tyrosinase maturation process, has scientific and industrial significance with respect to biochemical and technical applications of the enzyme. Here, not only the first crystal structure of the mushroom tyrosinase abPPO4 is presented in its active form (Ser2-Ser383) and in its 21 kDa heavier latent form (Ser2-Thr545), but furthermore the simultaneous presence of both forms within one single-crystal structure is shown. This allows for a simple approach to investigate the transition between these two forms. Isoform abPPO4 was isolated and extensively purified from the natural source (Agaricus bisporus), which contains a total of six polyphenol oxidases (PPOs). The enzyme formed crystals (diffracting to a resolution of 2.76 angstrom) owing to the employment of the 6-tungstotellurate(VI) salt (Na-6[TeW6O24]center dot 22H(2)O) as a cocrystallization agent. Two of these disc-shaped Anderson-type polyoxoanions [TeW6O24](6-) separate two asymmetric units comprising one crystallographic heterodimer of abPPO4, thus resulting in very interesting crystal packing.
机译：双官能近年,酪氨酸酶催化monophenols和o-diphenols的氧化邻醌的前体化合物这次黑色素。作为潜在的生物,酪氨酸酶表达发酵菌必须proteolytically裂解为了形成高活性的酶。激活机制,称为酪氨酸酶成熟过程中,科学与工业生化和意义技术应用的酶。只有第一个蘑菇的晶体结构酪氨酸酶abPPO4提出了其活性形式(Ser2-Ser383)和潜伏在其21 kDa重形式(Ser2-Thr545),但此外在一个同时存在的两种形式单晶结构。简单的方法探讨这两种形式之间的过渡。abPPO4是孤立的、广泛的纯化自然源(双孢菇)总共包含了六个多酚氧化酶类(ppo)。的分辨率2.76埃)由于就业的6-tungstotellurate (VI)盐共结晶剂。Anderson-type polyoxoanions (TeW6O24) (6)单独的两个不对称单元组成abPPO4晶体异质二聚体,从而导致非常有趣的水晶包装。

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