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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Structure and activity of the Streptococcus pyogenes family GH1 6-phospho-Β-glucosidase SPy1599
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Structure and activity of the Streptococcus pyogenes family GH1 6-phospho-Β-glucosidase SPy1599

机译：链球菌的结构和活动化脓性链球菌家庭gh 6-phospho——Β葡糖苷酶SPy1599

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摘要

The group A streptococcus Streptococcus pyogenes is the causative agent of a wide spectrum of invasive infections, including necrotizing fasciitis, scarlet fever and toxic shock syndrome. In the context of its carbohydrate chemistry, it is interesting that S. pyogenes (in this work strain M1 GAS SF370) displays a spectrum of oligosaccharide-processing enzymes that are located in close proximity on the genome but that the in vivo function of these proteins remains unknown. These proteins include different sugar transporters (SPy1593 and SPy1595), both GH125 -1,6- and GH38 -1,3-manno-sidases (SPy1603 and SPy1604), a GH84 Β-hexosaminidase (SPy1600) and a putative GH2 Β-galactosidase (SPy1586), as well as SPy1599, a family GH1 putative Β-glucosidase. Here, the solution of the three-dimensional structure of SPy1599 in a number of crystal forms complicated by unusual crystallo-graphic twinning is reported. The structure is a classical (Β/)8-barrel, consistent with CAZy family GH1 and other members of the GH-A clan. SPy1599 has been annotated in sequence depositions as a Β-glucosidase (EC 3.2.1.21), but no such activity could be found; instead, three-dimensional structural overlaps with other enzymes of known function suggested that SPy1599 contains a phosphate-binding pocket in the active site and has possible 6-phospho-Β-glycosidase activity. Subsequent kinetic analysis indeed showed that SPy1599 has 6-phospho-Β - glucosidase (EC 3.2.1.86) activity. These data suggest that SPy1599 is involved in the intracellular degradation of 6 - phosphoglycosides, which are likely to originate from import through one of the organisms many phosphoenolpyruvate phosphotransfer systems (PEP-PTSs).
机译：A组链球菌酿脓链球菌的病原体是一个广泛的吗侵入性感染,包括坏死筋膜炎、猩红热和中毒性休克并发症状化学,有趣的是,链球菌这项工作菌株M1气体SF370)显示oligosaccharide-processing酶的光谱位于附近的基因组但这些蛋白质的体内功能仍然是未知的。糖转运蛋白(SPy1593和SPy1595)GH125 1 6 -和GH38 1 3-manno-sidases (SPy1603和SPy1604),一个GH84Β己糖胺酶(SPy1600)和一个假定的GH2Β牛乳糖(SPy1586)SPy1599、gh假定的Β葡糖苷酶。SPy1599的三维结构数量的晶体形式复杂,不同寻常crystallo-graphic孪生。结构是一个经典(Β/)8-barrel,一致与CAZy家人gh和其他的成员了一家族。口供作为Β葡糖苷酶(EC 3.2.1.21),但是没有这样的活动能找到;三维结构重叠与其他建议SPy1599酶的功能包含一个phosphate-binding口袋里的活跃网站,有可能6-phospho——Β糖苷酶活动。表明SPy1599 6-phospho——Β-葡糖苷酶(EC 3.2.1.86)活动。SPy1599参与细胞内6 - phosphoglycosides退化,这是通过之一可能来自进口生物多磷酸烯醇丙酮酸phosphotransfer系统(PEP-PTSs)。

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