...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >The structure of a glycoside hydrolase family 81 endo-β-1,3-glucanase
【24h】

The structure of a glycoside hydrolase family 81 endo-β-1,3-glucanase

机译：81年糖苷水解酶家族的结构endo -β1,3-glucanase

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Endo-β-1,3-glucanases catalyze the hydrolysis of β-1,3-glycosidic linkages in glucans. They are also responsible for rather diverse physiological functions such as carbon utilization, cell-wall organization and pathogen defence. Glycoside hydrolase (GH) family 81 mainly consists of β-1,3-glucanases from fungi, higher plants and bacteria. A novel GH family 81 β-1,3-glucanase gene (RmLam81A) from Rhizomucor miehei was expressed in Escherichia coli. Purified RmLam81A was crystallized and the structure was determined in two crystal forms (form I-free and form II-Se) at 2.3 and 2.014;? resolution, respectively. Here, the crystal structure of a member of GH family 81 is reported for the first time. The structure of RmLam81A is greatly different from all endo-β-1,3-glucanase structures available in the Protein Data Bank. The overall structure of the RmLam81A monomer consists of an N-terminal β-sandwich domain, a C-terminal (/)6 domain and an additional domain between them. Glu553 and Glu557 are proposed to serve as the proton donor and basic catalyst, respectively, in a single-displacement mechanism. In addition, Tyr386, Tyr482 and Ser554 possibly contribute to both the position or the ionization state of the basic catalyst Glu557. The first crystal structure of a GH family 81 member will be helpful in the study of the GH family 81 proteins and endo-β-1,3-glucanases.
机译：Endo -β1,3-glucanases催化的水解β1在葡聚糖3-glycosidic联系。同时负责,而不同的生理功能如碳利用率、细胞壁组织和病原体的防御。81主要由水解酶(GH)的家庭β1,3-glucanases从真菌、植物和更高细菌。基因(RmLam81A)从Rhizomucor miehei大肠杆菌中表达。结晶和结构是决定在两种晶体形式(形式可以和形式II-Se)在2.3和2.014;?在这里,GH的成员的晶体结构家庭81年首次报道。RmLam81A结构大大不同endo -β1,3-glucanase结构中可用蛋白质数据银行。RmLam81A单体由氨基端β三明治域,c端(/)6域和一个额外的域。提出了Glu557作为质子供体和基本的催化剂,分别single-displacement机制。Tyr386, Tyr482 Ser554可能导致这两个的位置或电离状态基本Glu557催化剂。GH家族的81个成员的结构有助于研究的GH 81蛋白质家族与安多-β-1,3-glucanases .

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号